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Research Article


Immunogenicity of culture filtrated proteins and whole-cell killed-formalin of Listeria monocytogenes to induced cellular immune response in vivo

Hassan H.k. Al-bayati, Sultan Ahmed Abdullah, Thamer Jaddoa Shihab, Muthanna Sultan, Qusai Saleh Jumaa.


Abstract
Background:
Listeria monocytogenes (LM) is a life-threatening bacterium affecting many individuals worldwide, including elderly people, pregnant women, and immune-deficient patients.

Aim:
Whole-Cell Killed-Formalin of Listeria monocytogenes Antigens (WKLMAgs) and Listeria Culture Filtrated Proteins (LCFPs) against challenge-attenuated Listeria monocytogenes after two booster doses (0, and 14 days) of immunization act as an antigen activating a high level of IgG3, IgM, CXCL2, and IL-1 beta.

Methods:
Forty male rats were randomly assigned to four groups. The first group served as a control negative, while the second positive (+) control was inoculation orally 1 ml with virulent (1×107 colony forming unit CFU/ml) of Listeria monocytogenes on day 28. Whereas, the other two groups were injected with 1 ml Whole Killed Listeria Monocytogenes Antigen (WKLMAgs) and 1 ml Listeria culture filtrate proteins (LCFPs) in two subcutaneously (S/C) doses with day 14 intervals, with at day 28 a challenged effective dose (1×107 CFU/ml) of virulent Listeria monocytogenes. Serum blood parameters. During the 35 days, the euthanized animal histopathology studies were performed on the (spleen, liver, small intestine, and brain).

Results:
The current study indicated a significant difference between WKLMAgs and LCFPs for serological tests Immunoglobulin M, chemokine (C-X-C motif) ligand 2, Immunoglobulin G3 and Interleukin 1beta compared to both negative and positive control at P <0.001. Additionally, the WKLMAgs and LCFPs led to a decrease in the histopathological changes of organs such as (brain, spleen, liver, and intestine) compared to the positive control, which affected the organs with microgranuloma, with a pathological difference between the WKLMAgs and LCFPs compared to the negative control group.

Conclusion:
Both WKLMAgs and LCFPs are capable to be as a vaccine candidate antigen for the induction of protective immunity against Listeria monocytogenes.

Key words: Listeria monocytogenes, IgM, CXCL2, Vaccine design, PCR


 
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How to Cite this Article
Pubmed Style

Al-bayati HH, Abdullah SA, Shihab TJ, Sultan M, Jumaa QS. Immunogenicity of culture filtrated proteins and whole-cell killed-formalin of Listeria monocytogenes to induced cellular immune response in vivo. Open Vet J. 2024; 14(12): 3581-3598. doi:10.5455/OVJ.2024.v14.i12.40

Web Style

Al-bayati HH, Abdullah SA, Shihab TJ, Sultan M, Jumaa QS. Immunogenicity of culture filtrated proteins and whole-cell killed-formalin of Listeria monocytogenes to induced cellular immune response in vivo. https://www.openveterinaryjournal.com/?mno=224048 [Access: January 21, 2025]. doi:10.5455/OVJ.2024.v14.i12.40

AMA (American Medical Association) Style

Al-bayati HH, Abdullah SA, Shihab TJ, Sultan M, Jumaa QS. Immunogenicity of culture filtrated proteins and whole-cell killed-formalin of Listeria monocytogenes to induced cellular immune response in vivo. Open Vet J. 2024; 14(12): 3581-3598. doi:10.5455/OVJ.2024.v14.i12.40


Vancouver/ICMJE Style

Al-bayati HH, Abdullah SA, Shihab TJ, Sultan M, Jumaa QS. Immunogenicity of culture filtrated proteins and whole-cell killed-formalin of Listeria monocytogenes to induced cellular immune response in vivo. Open Vet J. (2024), [cited January 21, 2025]; 14(12): 3581-3598. doi:10.5455/OVJ.2024.v14.i12.40


Harvard Style

Al-bayati, H. H., Abdullah, . S. A., Shihab, . T. J., Sultan, . M. & Jumaa, . Q. S. (2024) Immunogenicity of culture filtrated proteins and whole-cell killed-formalin of Listeria monocytogenes to induced cellular immune response in vivo. Open Vet J, 14 (12), 3581-3598. doi:10.5455/OVJ.2024.v14.i12.40


Turabian Style

Al-bayati, Hassan H.k., Sultan Ahmed Abdullah, Thamer Jaddoa Shihab, Muthanna Sultan, and Qusai Saleh Jumaa. 2024. Immunogenicity of culture filtrated proteins and whole-cell killed-formalin of Listeria monocytogenes to induced cellular immune response in vivo. Open Veterinary Journal, 14 (12), 3581-3598. doi:10.5455/OVJ.2024.v14.i12.40


Chicago Style

Al-bayati, Hassan H.k., Sultan Ahmed Abdullah, Thamer Jaddoa Shihab, Muthanna Sultan, and Qusai Saleh Jumaa. "Immunogenicity of culture filtrated proteins and whole-cell killed-formalin of Listeria monocytogenes to induced cellular immune response in vivo." Open Veterinary Journal 14 (2024), 3581-3598. doi:10.5455/OVJ.2024.v14.i12.40


MLA (The Modern Language Association) Style

Al-bayati, Hassan H.k., Sultan Ahmed Abdullah, Thamer Jaddoa Shihab, Muthanna Sultan, and Qusai Saleh Jumaa. "Immunogenicity of culture filtrated proteins and whole-cell killed-formalin of Listeria monocytogenes to induced cellular immune response in vivo." Open Veterinary Journal 14.12 (2024), 3581-3598. Print. doi:10.5455/OVJ.2024.v14.i12.40


APA (American Psychological Association) Style

Al-bayati, H. H., Abdullah, . S. A., Shihab, . T. J., Sultan, . M. & Jumaa, . Q. S. (2024) Immunogenicity of culture filtrated proteins and whole-cell killed-formalin of Listeria monocytogenes to induced cellular immune response in vivo. Open Veterinary Journal, 14 (12), 3581-3598. doi:10.5455/OVJ.2024.v14.i12.40


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