E-ISSN 2218-6050 | ISSN 2226-4485
 
2025, Vol: 15, Issue: 2
15 / 2Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles

Research Article


Phylogenetic analysis and genetic evaluation of the Echinococcus granulosus antigen B subunit 2 gene isolated from sheep in Al-Diwaniyah, Iraq

Weam A. Hamad, Thanaa Ismael Jawad, Wegdan H. Atiya.


Abstract
Background:
Hydatid cysts are an immature form of Echinococcus granulosus tapeworm, which causes zoonotic illness, echinococcosis (sometimes called hydatidosis). There are 10 officially recognized genotypes (G1-G10) of this parasite.

Aim:
The current study aimed to sequence and characterize the genotypes of E. granulosus using the antigen B subunit 2 gene isolated from sheep in Al-Diwaniyah City, Iraq.

Methods:
Using specific antigen B subunit-2, 50 hydatid cysts were collected from the livers of sheep at a local slaughterhouse between March and October 2023. DNA was obtained from the hydatid fluid of sheep cysts. Protescolices from fertile cyst fluid were also obtained. Conventional PCR targeting the Antigen B gene was performed on the extracted DNA of samples. PCR was performed followed by sequencing and phylogenetic analysis of PCR 7 positive isolates.

Results:
The predominant genotype in the sheep population was the G1 strain. Furthermore, the sequenced isolates exhibited a high degree of correlation with local and regional Iran E. granulosus sequences, demonstrating genetic diversity compared with reference strains. The current isolates were assigned accession numbers by the NCBI as follows: OR232318.1, OR232319.1, OR232320.1, OR232321.1, OR232322.1, OR232323.1, and OR232324.1.

Conclusion:
The findings indicate that E. granulosus has diverse genotypes and is mostly linked genetically to isolates from neighboring countries, such as Brazil and Iraq.

Key words: Echinococcus granulosus, Parasite, Sheep


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Weam A. Hamad
Articles by Thanaa Ismael Jawad
Articles by Wegdan H. Atiya
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Hamad WA, Jawad TI, Atiya WH. Phylogenetic analysis and genetic evaluation of the Echinococcus granulosus antigen B subunit 2 gene isolated from sheep in Al-Diwaniyah, Iraq. Open Vet J. 2025; 15(2): 1043-1049. doi:10.5455/OVJ.2025.v15.i2.52

Web Style

Hamad WA, Jawad TI, Atiya WH. Phylogenetic analysis and genetic evaluation of the Echinococcus granulosus antigen B subunit 2 gene isolated from sheep in Al-Diwaniyah, Iraq. https://www.openveterinaryjournal.com/?mno=226126 [Access: March 13, 2025]. doi:10.5455/OVJ.2025.v15.i2.52

AMA (American Medical Association) Style

Hamad WA, Jawad TI, Atiya WH. Phylogenetic analysis and genetic evaluation of the Echinococcus granulosus antigen B subunit 2 gene isolated from sheep in Al-Diwaniyah, Iraq. Open Vet J. 2025; 15(2): 1043-1049. doi:10.5455/OVJ.2025.v15.i2.52


Vancouver/ICMJE Style

Hamad WA, Jawad TI, Atiya WH. Phylogenetic analysis and genetic evaluation of the Echinococcus granulosus antigen B subunit 2 gene isolated from sheep in Al-Diwaniyah, Iraq. Open Vet J. (2025), [cited March 13, 2025]; 15(2): 1043-1049. doi:10.5455/OVJ.2025.v15.i2.52


Harvard Style

Hamad, W. A., Jawad, . T. I. & Atiya, . W. H. (2025) Phylogenetic analysis and genetic evaluation of the Echinococcus granulosus antigen B subunit 2 gene isolated from sheep in Al-Diwaniyah, Iraq. Open Vet J, 15 (2), 1043-1049. doi:10.5455/OVJ.2025.v15.i2.52


Turabian Style

Hamad, Weam A., Thanaa Ismael Jawad, and Wegdan H. Atiya. 2025. Phylogenetic analysis and genetic evaluation of the Echinococcus granulosus antigen B subunit 2 gene isolated from sheep in Al-Diwaniyah, Iraq. Open Veterinary Journal, 15 (2), 1043-1049. doi:10.5455/OVJ.2025.v15.i2.52


Chicago Style

Hamad, Weam A., Thanaa Ismael Jawad, and Wegdan H. Atiya. "Phylogenetic analysis and genetic evaluation of the Echinococcus granulosus antigen B subunit 2 gene isolated from sheep in Al-Diwaniyah, Iraq." Open Veterinary Journal 15 (2025), 1043-1049. doi:10.5455/OVJ.2025.v15.i2.52


MLA (The Modern Language Association) Style

Hamad, Weam A., Thanaa Ismael Jawad, and Wegdan H. Atiya. "Phylogenetic analysis and genetic evaluation of the Echinococcus granulosus antigen B subunit 2 gene isolated from sheep in Al-Diwaniyah, Iraq." Open Veterinary Journal 15.2 (2025), 1043-1049. Print. doi:10.5455/OVJ.2025.v15.i2.52


APA (American Psychological Association) Style

Hamad, W. A., Jawad, . T. I. & Atiya, . W. H. (2025) Phylogenetic analysis and genetic evaluation of the Echinococcus granulosus antigen B subunit 2 gene isolated from sheep in Al-Diwaniyah, Iraq. Open Veterinary Journal, 15 (2), 1043-1049. doi:10.5455/OVJ.2025.v15.i2.52


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense InformationCopyright Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldagpublisher.com 

Email: ibrahim.eldaghayes@vetmed.edu.ly