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2025, Vol: 15, Issue: 3
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Research Article


Effectiveness of predatory bacterium Bdellovibrio bacteriovorus in combination with Pseudomonas fluorescens and Lactobacillus acidophilus as candidates for in vitro anticolibacillosis

Eduardus Bimo Aksono, Muchammad Yunus, Iwan Sahrial Hamid, Gadis Meinar Sari.


Abstract
Background:
Bdellovibrio bacteriovorus has stood out since its initial discovery because of its exceptional capacity to feed on other Gram-negative bacteria. Since this specific “predatory bacterium” may be used as both a probiotic and an antibiotic, research on it has expanded in response to the growing issue of AMR. It is necessary to investigate the relationship between B. bacteriovorus and other Gram-negative bacteria, as well as the presence of Gram-positive bacteria, in the same environment to determine whether or not these factors have an impact on their ability as predators.

Aim:
This study aimed to assess the effectiveness of the combination of the predatory bacterium B. bacteriovorus with Pseudomonas fluorescens and Lactobacillus acidophilus as potential candidates for in vitro anticolibacillosis.

Methods:
The method employed Escherichia coli ATCC 15144 as the prey, while B. bacteriovorus 109 J ATCC 15143 was used as the predator, combined with P. fluorescens and L. acidophilus as the nutrient sources of the predator. In the challenge experiment, a ratio of the bacterial combination was used to optimize predation to E. coli of 107 PFU/105 CFU per ml.

Results:
The study showed that the combination of B. bacteriovorus with P. fluorescens and L. acidophilus after 24 hours of in vitro incubation at 37°C increased the predatory bacteria count by tenfold, effectively reducing the E. coli population. However, in the absence of Gram-negative bacteria as a nutrient source, the predator population gradually declined.

Conclusion:
The combination of B. bacteriovorus as a predatory bacterium with P. fluorescens and L. acidophilus is an effective candidate for in vitro anticolibacillosis.

Key words: B. bacteriovorus, P. fluorescens, L. acidophilus, Anticolibacillosis, in vitro


 
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How to Cite this Article
Pubmed Style

Aksono EB, Yunus M, Hamid IS, Sari GM. Effectiveness of predatory bacterium Bdellovibrio bacteriovorus in combination with Pseudomonas fluorescens and Lactobacillus acidophilus as candidates for in vitro anticolibacillosis. Open Vet J. 2025; 15(3): 1379-1386. doi:10.5455/OVJ.2025.v15.i3.29

Web Style

Aksono EB, Yunus M, Hamid IS, Sari GM. Effectiveness of predatory bacterium Bdellovibrio bacteriovorus in combination with Pseudomonas fluorescens and Lactobacillus acidophilus as candidates for in vitro anticolibacillosis. https://www.openveterinaryjournal.com/?mno=234167 [Access: April 09, 2025]. doi:10.5455/OVJ.2025.v15.i3.29

AMA (American Medical Association) Style

Aksono EB, Yunus M, Hamid IS, Sari GM. Effectiveness of predatory bacterium Bdellovibrio bacteriovorus in combination with Pseudomonas fluorescens and Lactobacillus acidophilus as candidates for in vitro anticolibacillosis. Open Vet J. 2025; 15(3): 1379-1386. doi:10.5455/OVJ.2025.v15.i3.29


Vancouver/ICMJE Style

Aksono EB, Yunus M, Hamid IS, Sari GM. Effectiveness of predatory bacterium Bdellovibrio bacteriovorus in combination with Pseudomonas fluorescens and Lactobacillus acidophilus as candidates for in vitro anticolibacillosis. Open Vet J. (2025), [cited April 09, 2025]; 15(3): 1379-1386. doi:10.5455/OVJ.2025.v15.i3.29


Harvard Style

Aksono, E. B., Yunus, . M., Hamid, . I. S. & Sari, . G. M. (2025) Effectiveness of predatory bacterium Bdellovibrio bacteriovorus in combination with Pseudomonas fluorescens and Lactobacillus acidophilus as candidates for in vitro anticolibacillosis. Open Vet J, 15 (3), 1379-1386. doi:10.5455/OVJ.2025.v15.i3.29


Turabian Style

Aksono, Eduardus Bimo, Muchammad Yunus, Iwan Sahrial Hamid, and Gadis Meinar Sari. 2025. Effectiveness of predatory bacterium Bdellovibrio bacteriovorus in combination with Pseudomonas fluorescens and Lactobacillus acidophilus as candidates for in vitro anticolibacillosis. Open Veterinary Journal, 15 (3), 1379-1386. doi:10.5455/OVJ.2025.v15.i3.29


Chicago Style

Aksono, Eduardus Bimo, Muchammad Yunus, Iwan Sahrial Hamid, and Gadis Meinar Sari. "Effectiveness of predatory bacterium Bdellovibrio bacteriovorus in combination with Pseudomonas fluorescens and Lactobacillus acidophilus as candidates for in vitro anticolibacillosis." Open Veterinary Journal 15 (2025), 1379-1386. doi:10.5455/OVJ.2025.v15.i3.29


MLA (The Modern Language Association) Style

Aksono, Eduardus Bimo, Muchammad Yunus, Iwan Sahrial Hamid, and Gadis Meinar Sari. "Effectiveness of predatory bacterium Bdellovibrio bacteriovorus in combination with Pseudomonas fluorescens and Lactobacillus acidophilus as candidates for in vitro anticolibacillosis." Open Veterinary Journal 15.3 (2025), 1379-1386. Print. doi:10.5455/OVJ.2025.v15.i3.29


APA (American Psychological Association) Style

Aksono, E. B., Yunus, . M., Hamid, . I. S. & Sari, . G. M. (2025) Effectiveness of predatory bacterium Bdellovibrio bacteriovorus in combination with Pseudomonas fluorescens and Lactobacillus acidophilus as candidates for in vitro anticolibacillosis. Open Veterinary Journal, 15 (3), 1379-1386. doi:10.5455/OVJ.2025.v15.i3.29


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