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Research Article 


Morphometrics Analysis and Molecular Identification of the Trypanozoon from Indonesia

Didik Tulus Subekti, Lucia Tri Suwanti, Sunarno Sunarno, Mufasirin Mufasirin, Endang Suprihati, Iwan Sahrial Hamid, Eduardus Bimo Aksono Herupradoto, Emy Koestanti Sabdoningrum.


Abstract
Background: Morphologically, the species within the subgenus Trypanozoon cannot be clearly distinguished.
AIM: To identify and characterize trypanosome species in Indonesia through molecular algorithm that was carried out in stages using four pairs of primers to confirm and distinguish between species of the subgenus Trypanozoon.
Methods: A total of 28 trypanosome isolates were examined microscopically and their morphobiometrics were observed. The isolates were also identified molecularly with an identification algorithm involving 4 pairs of primers in a hierarchical manner namely ITS1, ESAG6/7, minicircle and maxicircle.
Results: Morphologically, Indonesian trypanosomes are characterized by a long slender shape, small kinetoplasts located below the posterior terminal end, and free flagella with conspicuous undulating membranes. Screening using ITS1 and ESAG6/7 showed a single band at 480 bp and 237 bp which indicating the subgenus Trypanozoon Species identification using minicircle primer followed by maxicircle primer showed that Indonesian trypanosomes are Trypanosoma (Trypanozoon) evansi (T. evansi) and Trypanosoma (Trypanozoon) equiperdum (T. equiperdum). Indonesian T. evansi has a total body length of 26.45 μm (22.79-29.04 μm) with the kinetoplast positioned at 1.73 μm from the posterior end, while Indonesian T. equiperdum has a body length of 26.41 μm (22.83-30.89 μm) and the kinetoplast positioned at 1.91 μm from the posterior end.
Conclusions: Molecular identification algorithms can distinguish species of the subgenus Trypanozoon more accurately than morphobiometric observations. Trypanozoon species found in Indonesia include Trypanosoma evansi and Trypanosoma equiperdum.
Clinical relevance: The minicircle and maxicircle primers are the key to species identification of Trapanozoon which can be applied to overcome the identification difficulties that have been experienced so far.

Key words: algorithms, molecular identification, morphometric, Trypanosoma evansi, Trypanosoma equiperdum


 
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How to Cite this Article
Pubmed Style

Subekti DT, Suwanti LT, Sunarno S, Mufasirin M, Suprihati E, Hamid IS, Herupradoto EBA, Sabdoningrum EK. Morphometrics Analysis and Molecular Identification of the Trypanozoon from Indonesia. Open Vet J. 2025; 15(7): 3254-3268. doi:10.5455/OVJ.2025.v15.i7.36

Web Style

Subekti DT, Suwanti LT, Sunarno S, Mufasirin M, Suprihati E, Hamid IS, Herupradoto EBA, Sabdoningrum EK. Morphometrics Analysis and Molecular Identification of the Trypanozoon from Indonesia. https://www.openveterinaryjournal.com/?mno=240539 [Access: August 15, 2025]. doi:10.5455/OVJ.2025.v15.i7.36

AMA (American Medical Association) Style

Subekti DT, Suwanti LT, Sunarno S, Mufasirin M, Suprihati E, Hamid IS, Herupradoto EBA, Sabdoningrum EK. Morphometrics Analysis and Molecular Identification of the Trypanozoon from Indonesia. Open Vet J. 2025; 15(7): 3254-3268. doi:10.5455/OVJ.2025.v15.i7.36


Vancouver/ICMJE Style

Subekti DT, Suwanti LT, Sunarno S, Mufasirin M, Suprihati E, Hamid IS, Herupradoto EBA, Sabdoningrum EK. Morphometrics Analysis and Molecular Identification of the Trypanozoon from Indonesia. Open Vet J. (2025), [cited August 15, 2025]; 15(7): 3254-3268. doi:10.5455/OVJ.2025.v15.i7.36


Harvard Style

Subekti, D. T., Suwanti, . L. T., Sunarno, . S., Mufasirin, . M., Suprihati, . E., Hamid, . I. S., Herupradoto, . E. B. A. & Sabdoningrum, . E. K. (2025) Morphometrics Analysis and Molecular Identification of the Trypanozoon from Indonesia. Open Vet J, 15 (7), 3254-3268. doi:10.5455/OVJ.2025.v15.i7.36


Turabian Style

Subekti, Didik Tulus, Lucia Tri Suwanti, Sunarno Sunarno, Mufasirin Mufasirin, Endang Suprihati, Iwan Sahrial Hamid, Eduardus Bimo Aksono Herupradoto, and Emy Koestanti Sabdoningrum. 2025. Morphometrics Analysis and Molecular Identification of the Trypanozoon from Indonesia. Open Veterinary Journal, 15 (7), 3254-3268. doi:10.5455/OVJ.2025.v15.i7.36


Chicago Style

Subekti, Didik Tulus, Lucia Tri Suwanti, Sunarno Sunarno, Mufasirin Mufasirin, Endang Suprihati, Iwan Sahrial Hamid, Eduardus Bimo Aksono Herupradoto, and Emy Koestanti Sabdoningrum. "Morphometrics Analysis and Molecular Identification of the Trypanozoon from Indonesia." Open Veterinary Journal 15 (2025), 3254-3268. doi:10.5455/OVJ.2025.v15.i7.36


MLA (The Modern Language Association) Style

Subekti, Didik Tulus, Lucia Tri Suwanti, Sunarno Sunarno, Mufasirin Mufasirin, Endang Suprihati, Iwan Sahrial Hamid, Eduardus Bimo Aksono Herupradoto, and Emy Koestanti Sabdoningrum. "Morphometrics Analysis and Molecular Identification of the Trypanozoon from Indonesia." Open Veterinary Journal 15.7 (2025), 3254-3268. Print. doi:10.5455/OVJ.2025.v15.i7.36


APA (American Psychological Association) Style

Subekti, D. T., Suwanti, . L. T., Sunarno, . S., Mufasirin, . M., Suprihati, . E., Hamid, . I. S., Herupradoto, . E. B. A. & Sabdoningrum, . E. K. (2025) Morphometrics Analysis and Molecular Identification of the Trypanozoon from Indonesia. Open Veterinary Journal, 15 (7), 3254-3268. doi:10.5455/OVJ.2025.v15.i7.36


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