E-ISSN 2218-6050 | ISSN 2226-4485
 
2025, Vol: 15, Issue: 4
15 / 4Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submissio Form

Research Article 


The beneficial impact of oregano essential oil supplementation on growth, physiological function, antioxidant activity, and immune response in Nile tilapia (Oreochromis niloticus) fingerlings under Aeromonas hydrophila stress

Mohamed A. Khallaf, Wesam H. Marzouk, Hanan A. Ghetas, Haguer M. Salah El Din, Mona Assas, Azza Hafez, Alaa Elgaabari, Eman M. Moustafa.


Abstract
Background:
Adding oregano essential oil (OEO) as a supplement has great potential to improve fish production efficiency.

Aim:
Current research was executed to assess the prospective impact of OEO incorporation into tilapia diets on physiological response, growth performance, oxidative capacity, and immune status when subjected to Aeromonas hydrophila infection.

Methods:
Three different experimental diets were prepared by augmenting a 30% protein basal diet with OEO at concentrations of 0, 0.5, and 1 g/kg feed. Each of the three treatment groups consisted of sixty fish (three copies of twenty fish each) and was randomly assigned out of 180 healthy Nile tilapia (O. niloticus) fingerlings. For 8 weeks, fish in nine separate glass tanks were given individualized diets containing 3% biomass. The survival rate (SR%) of the fish was measured after 15 days of observation following intraperitoneal injection of pathogenic A. hydrophila.

Results:
Physiological performance, antioxidant indicators, immunological function (phagocytic activity, lysozyme, and phagocytic index), and growth metrics were all enhanced in fish that were given OEO supplements. Antibiotics may be a new contender: oregano oil, when consumed orally, reduces pathogenic oxidative stress. The villus height, breadth, and depth were enhanced by oregano oil, which also improved intestinal morphology. On the other hand, animals infected with A. hydrophila exhibited signs of deterioration in the spleen, hepatopancreas, and intestinal tissues.

Conclusion:
For optimal development, immunity, performance in physiological tests, and gut health, Nile tilapia fingerlings are fed half a gram per kilogram of OEO.

Key words: Keywords: Aeromonas hydrophila, Nile tilapia, Oregano essential oil, Oxidative status


 
ARTICLE TOOLS
Abstract
PDF Fulltext
HTML Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Mohamed A. Khallaf
Articles by Wesam H. Marzouk
Articles by Hanan A. Ghetas
Articles by Haguer M. Salah El Din
Articles by Mona Assas
Articles by Azza Hafez
Articles by Alaa Elgaabari
Articles by Eman M. Moustafa
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Khallaf MA, Marzouk WH, Ghetas HA, Din HMSE, Assas M, Hafez A, Elgaabari A, Moustafa EM. The beneficial impact of oregano essential oil supplementation on growth, physiological function, antioxidant activity, and immune response in Nile tilapia (Oreochromis niloticus) fingerlings under Aeromonas hydrophila stress. Open Vet J. 2025; 15(3): 1424-1439. doi:10.5455/OVJ.2025.v15.i3.33

Web Style

Khallaf MA, Marzouk WH, Ghetas HA, Din HMSE, Assas M, Hafez A, Elgaabari A, Moustafa EM. The beneficial impact of oregano essential oil supplementation on growth, physiological function, antioxidant activity, and immune response in Nile tilapia (Oreochromis niloticus) fingerlings under Aeromonas hydrophila stress. https://www.openveterinaryjournal.com/?mno=248809 [Access: May 30, 2025]. doi:10.5455/OVJ.2025.v15.i3.33

AMA (American Medical Association) Style

Khallaf MA, Marzouk WH, Ghetas HA, Din HMSE, Assas M, Hafez A, Elgaabari A, Moustafa EM. The beneficial impact of oregano essential oil supplementation on growth, physiological function, antioxidant activity, and immune response in Nile tilapia (Oreochromis niloticus) fingerlings under Aeromonas hydrophila stress. Open Vet J. 2025; 15(3): 1424-1439. doi:10.5455/OVJ.2025.v15.i3.33


Vancouver/ICMJE Style

Khallaf MA, Marzouk WH, Ghetas HA, Din HMSE, Assas M, Hafez A, Elgaabari A, Moustafa EM. The beneficial impact of oregano essential oil supplementation on growth, physiological function, antioxidant activity, and immune response in Nile tilapia (Oreochromis niloticus) fingerlings under Aeromonas hydrophila stress. Open Vet J. (2025), [cited May 30, 2025]; 15(3): 1424-1439. doi:10.5455/OVJ.2025.v15.i3.33


Harvard Style

Khallaf, M. A., Marzouk, . W. H., Ghetas, . H. A., Din, . H. M. S. E., Assas, . M., Hafez, . A., Elgaabari, . A. & Moustafa, . E. M. (2025) The beneficial impact of oregano essential oil supplementation on growth, physiological function, antioxidant activity, and immune response in Nile tilapia (Oreochromis niloticus) fingerlings under Aeromonas hydrophila stress. Open Vet J, 15 (3), 1424-1439. doi:10.5455/OVJ.2025.v15.i3.33


Turabian Style

Khallaf, Mohamed A., Wesam H. Marzouk, Hanan A. Ghetas, Haguer M. Salah El Din, Mona Assas, Azza Hafez, Alaa Elgaabari, and Eman M. Moustafa. 2025. The beneficial impact of oregano essential oil supplementation on growth, physiological function, antioxidant activity, and immune response in Nile tilapia (Oreochromis niloticus) fingerlings under Aeromonas hydrophila stress. Open Veterinary Journal, 15 (3), 1424-1439. doi:10.5455/OVJ.2025.v15.i3.33


Chicago Style

Khallaf, Mohamed A., Wesam H. Marzouk, Hanan A. Ghetas, Haguer M. Salah El Din, Mona Assas, Azza Hafez, Alaa Elgaabari, and Eman M. Moustafa. "The beneficial impact of oregano essential oil supplementation on growth, physiological function, antioxidant activity, and immune response in Nile tilapia (Oreochromis niloticus) fingerlings under Aeromonas hydrophila stress." Open Veterinary Journal 15 (2025), 1424-1439. doi:10.5455/OVJ.2025.v15.i3.33


MLA (The Modern Language Association) Style

Khallaf, Mohamed A., Wesam H. Marzouk, Hanan A. Ghetas, Haguer M. Salah El Din, Mona Assas, Azza Hafez, Alaa Elgaabari, and Eman M. Moustafa. "The beneficial impact of oregano essential oil supplementation on growth, physiological function, antioxidant activity, and immune response in Nile tilapia (Oreochromis niloticus) fingerlings under Aeromonas hydrophila stress." Open Veterinary Journal 15.3 (2025), 1424-1439. Print. doi:10.5455/OVJ.2025.v15.i3.33


APA (American Psychological Association) Style

Khallaf, M. A., Marzouk, . W. H., Ghetas, . H. A., Din, . H. M. S. E., Assas, . M., Hafez, . A., Elgaabari, . A. & Moustafa, . E. M. (2025) The beneficial impact of oregano essential oil supplementation on growth, physiological function, antioxidant activity, and immune response in Nile tilapia (Oreochromis niloticus) fingerlings under Aeromonas hydrophila stress. Open Veterinary Journal, 15 (3), 1424-1439. doi:10.5455/OVJ.2025.v15.i3.33


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense InformationCopyright Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldaghayes.com 

Email: ibrahim.eldaghayes@vetmed.edu.ly