Abstract
Background:
This study was conducted to investigate the biochemical impact of acetyl-L-carnitine (350 mg/ewe) administration with or without Selenium-fortified Saccharomyces cerevisiae (0.03 mg/kg body weight) on 24 mature ewes for 60 days.

Aim:
This study aimed to investigate the biophysiological changes in some biochemical traits by adopting acetyl-L-carnitine with or without Selenium-fortified Saccharomyces cerevisiae and their combination.

Method:
This study investigated the effect of acetyl-L-carnitine (350 mg/ewe) with or without Selenium-fortified Saccharomyces cerevisiae (0.03 mg/kg body weight) on 24 mature ewes for 60 days.

Results:
This study investigated the biochemical impact of acetyl-l-carnitine and selenium-fortified Saccharomyces cerevisiae (Se-SC) on the metabolic and liver health of Awassi ewes over 60 days. A total of 24 mature ewes were randomly assigned to four treatment groups: a control group (no additives), a group receiving L-Carnitine (350 mg/ewe), a group receiving Selenium-fortified Saccharomyces cerevisiae (0.03 mg/kg BW), and a combined treatment group (L-Carnitine and Se-SC). The study focused on key biochemical parameters, including blood glucose, total protein, albumin, liver enzymes (AST and ALT), cholesterol, and triglycerides. The combined treatment (T4) significantly improved metabolic and liver health, reducing blood glucose, cholesterol, triglycerides, and liver enzymes, while enhancing total protein and albumin levels. Individual treatments also showed beneficial effects, with Se-SC enhancing protein metabolism and L-Carnitine optimizing lipid and glucose levels. These findings suggest that the synergistic effects of Acetyl-L-Carnitine and Se-SC may offer a promising strategy for improving metabolic regulation and liver function in ruminants, particularly in livestock management practices aiming for improved health and productivity.

Conclusion:
Both acetyl-L-carnitine and selenium-fortified Saccharomyces cerevisiae improved metabolic and liver health in ewes, with the combined treatment being the most effective.

Key words: Acetyl-L-carnitine; Saccharomyces cerevisiae; Selenium; Biochemical; Sheep.