E-ISSN 2218-6050 | ISSN 2226-4485
 

Original Article


Characterization of microbial communities and predicted metabolic pathways in the uterus of healthy mares

Pamela Thomson, Josefina Pareja, Andrea Núñez, Rodrigo Santibáñez, Rodrigo Castro.

Abstract
Background: Culture-independent techniques have made it possible to expand the knowledge about the composition of bacterial communities present in the healthy uterus and their role in health and disease, mainly in humans. However, in animals like mares, there is a dearth of information regarding this area.
Aim: To narrow this knowledge gap, the objective of this study was to identify and characterize the composition and function for the uterine microbiome of a group of Chilean purebred mares (CPM), an equine breed with the oldest genealogical record in South America and an economical important reproductive industry.
Methods: From uterine biopsy samples obtained during estrus, DNA extraction and targeted sequencing was performed to investigate the bacterial diversity and its probable metabolic function.
Results: CPM biopsy samples were characterized by having a varied microbial composition, where the four most relatively abundant phyla were Proteobacteria (69.6%), Firmicutes (21.1%), Bacteroidetes (7.8%), and Actinobacteria (1.06%); which made up 99.6% of the total identified phyla. In contrast, Actinobacteria and Fusobacteria were the phyla not identified in all samples. Of a total of 59 genera identified across all samples, Staphylococcus was the most abundant genus with an average relative abundance of 18.88%, followed by Pseudomonas (17.9%), Escherichia/Shigella (10.42%), and Klebsiella (9.92%).
Conclusion: These findings contribute to the knowledge of microbes presence in the uterus, while future studies are required to demonstrate the role of these microorganisms in health and disease.

Key words: uterine microbes, mare, metabolic pathways


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Pamela Thomson
Articles by Josefina Pareja
Articles by Andrea Núñez
Articles by Rodrigo Santibáñez
Articles by Rodrigo Castro
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Thomson P, Pareja J, AN, Santibáñez R, Castro R. Characterization of microbial communities and predicted metabolic pathways in the uterus of healthy mares. Open Vet J. 2022; 12(6): 797-805. doi:10.5455/OVJ.2022.v12.i6.3


Web Style

Thomson P, Pareja J, AN, Santibáñez R, Castro R. Characterization of microbial communities and predicted metabolic pathways in the uterus of healthy mares. https://www.openveterinaryjournal.com/?mno=26153 [Access: November 28, 2022]. doi:10.5455/OVJ.2022.v12.i6.3


AMA (American Medical Association) Style

Thomson P, Pareja J, AN, Santibáñez R, Castro R. Characterization of microbial communities and predicted metabolic pathways in the uterus of healthy mares. Open Vet J. 2022; 12(6): 797-805. doi:10.5455/OVJ.2022.v12.i6.3



Vancouver/ICMJE Style

Thomson P, Pareja J, AN, Santibáñez R, Castro R. Characterization of microbial communities and predicted metabolic pathways in the uterus of healthy mares. Open Vet J. (2022), [cited November 28, 2022]; 12(6): 797-805. doi:10.5455/OVJ.2022.v12.i6.3



Harvard Style

Thomson, P., Pareja, . J., , . A. N., Santibáñez, . R. & Castro, . R. (2022) Characterization of microbial communities and predicted metabolic pathways in the uterus of healthy mares. Open Vet J, 12 (6), 797-805. doi:10.5455/OVJ.2022.v12.i6.3



Turabian Style

Thomson, Pamela, Josefina Pareja, Andrea Núñez, Rodrigo Santibáñez, and Rodrigo Castro. 2022. Characterization of microbial communities and predicted metabolic pathways in the uterus of healthy mares. Open Veterinary Journal, 12 (6), 797-805. doi:10.5455/OVJ.2022.v12.i6.3



Chicago Style

Thomson, Pamela, Josefina Pareja, Andrea Núñez, Rodrigo Santibáñez, and Rodrigo Castro. "Characterization of microbial communities and predicted metabolic pathways in the uterus of healthy mares." Open Veterinary Journal 12 (2022), 797-805. doi:10.5455/OVJ.2022.v12.i6.3



MLA (The Modern Language Association) Style

Thomson, Pamela, Josefina Pareja, Andrea Núñez, Rodrigo Santibáñez, and Rodrigo Castro. "Characterization of microbial communities and predicted metabolic pathways in the uterus of healthy mares." Open Veterinary Journal 12.6 (2022), 797-805. Print. doi:10.5455/OVJ.2022.v12.i6.3



APA (American Psychological Association) Style

Thomson, P., Pareja, . J., , . A. N., Santibáñez, . R. & Castro, . R. (2022) Characterization of microbial communities and predicted metabolic pathways in the uterus of healthy mares. Open Veterinary Journal, 12 (6), 797-805. doi:10.5455/OVJ.2022.v12.i6.3