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2025, Vol: 15, Issue: 9
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Research Article


Impact of varying L-carnitine doses on the motility and activation parameters of bull sperm in vitro during low-motile sperm cryopreservation

Ahmed Saed Al-ebady.


Abstract
Background:
L-carnitine is an important amino acid in sperm metabolism and possesses antioxidant properties. It makes energy easy for spermatozoa to access, which helps in sperm movement, and has the ‎effect of decreasing reactive oxygen species production, which helps in lessening their harmful ‎effects.‎

Aim:
To investigate the optimal dose of L-carnitine to be used in bull semen extenders for mitigating the harmful effects of reactive oxygen species during semen cryopreservation.

Methods:
A total of 38 ejaculates (30-55% motility) were collected and extended using a Tris-yolk-fructose extender. Each sample was divided into five groups: a control and four groups supplemented with L-carnitine (0.03, 0.05, 0.08, and 0.1 mg/ml). Sperm motility, viability, and abnormalities were assessed before and after cryopreservation.

Results:
Supplementation of the semen extender with L-carnitine for low-motile bull sperm significantly improved sperm motility, with the optimal concentration being 0.05 mg/ml (P<0.05). Additionally, L-carnitine reduced sperm abnormalities and mortality post-thaw. These findings indicate that L-carnitine can be a valuable semen extender supplement to enhance sperm preservation during cryopreservation.

Conclusion:
Both before and after cryopreservation, certain sperm functional parameters improved following in vitro bull sperm activation with L-carnitine-enriched media.

Key words: L-carnitine; Poor motile bull sperm; Cryopreservation; Sperm activation.


 
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Pubmed Style

Ahmed Saed Al-Ebady. Impact of varying L-carnitine doses on the motility and activation parameters of bull sperm in vitro during low-motile sperm cryopreservation. Open Vet. J.. 2025; 15(9): 4199-4203. doi:10.5455/OVJ.2025.v15.i9.25

Web Style

Ahmed Saed Al-Ebady. Impact of varying L-carnitine doses on the motility and activation parameters of bull sperm in vitro during low-motile sperm cryopreservation. https://www.openveterinaryjournal.com/?mno=262138 [Access: October 06, 2025]. doi:10.5455/OVJ.2025.v15.i9.25

AMA (American Medical Association) Style

Ahmed Saed Al-Ebady. Impact of varying L-carnitine doses on the motility and activation parameters of bull sperm in vitro during low-motile sperm cryopreservation. Open Vet. J.. 2025; 15(9): 4199-4203. doi:10.5455/OVJ.2025.v15.i9.25


Vancouver/ICMJE Style

Ahmed Saed Al-Ebady. Impact of varying L-carnitine doses on the motility and activation parameters of bull sperm in vitro during low-motile sperm cryopreservation. Open Vet. J.. (2025), [cited October 06, 2025]; 15(9): 4199-4203. doi:10.5455/OVJ.2025.v15.i9.25


Harvard Style

Ahmed Saed Al-Ebady (2025) Impact of varying L-carnitine doses on the motility and activation parameters of bull sperm in vitro during low-motile sperm cryopreservation. Open Vet. J., 15 (9), 4199-4203. doi:10.5455/OVJ.2025.v15.i9.25


Turabian Style

Ahmed Saed Al-Ebady. 2025. Impact of varying L-carnitine doses on the motility and activation parameters of bull sperm in vitro during low-motile sperm cryopreservation. Open Veterinary Journal, 15 (9), 4199-4203. doi:10.5455/OVJ.2025.v15.i9.25


Chicago Style

Ahmed Saed Al-Ebady. "Impact of varying L-carnitine doses on the motility and activation parameters of bull sperm in vitro during low-motile sperm cryopreservation." Open Veterinary Journal 15 (2025), 4199-4203. doi:10.5455/OVJ.2025.v15.i9.25


MLA (The Modern Language Association) Style

Ahmed Saed Al-Ebady. "Impact of varying L-carnitine doses on the motility and activation parameters of bull sperm in vitro during low-motile sperm cryopreservation." Open Veterinary Journal 15.9 (2025), 4199-4203. Print. doi:10.5455/OVJ.2025.v15.i9.25


APA (American Psychological Association) Style

Ahmed Saed Al-Ebady (2025) Impact of varying L-carnitine doses on the motility and activation parameters of bull sperm in vitro during low-motile sperm cryopreservation. Open Veterinary Journal, 15 (9), 4199-4203. doi:10.5455/OVJ.2025.v15.i9.25


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