E-ISSN 2218-6050 | ISSN 2226-4485
 
2025, Vol: 15, Issue: 11
15 / 11Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submissio Form
mainarticle_template

Research Article


Molecular and histopathological investigation about the impact of Zuhdi Date palm (Phoenix dactylifera L.) seeds on infected and healthy female rats

Maha Abdul-hadi Abdul-rida Al-abdula, Saffia Kareem Wally Alumeri, Eman F. Albaghdady.


Abstract
Background:
Date palm (Phoenix dactylifera L.) seeds are rich in bioactive compounds with known antioxidant, anti-inflammatory, and antimicrobial properties. Despite their growing use in traditional medicine, their impact on intestinal health remains underexplored.

Aim:
This study examined the molecular and histopathological effects of Zuhdi date seed extract on the gastrointestinal tract of infected and healthy female rats.

Methods:
A total of 40 albino rats were divided into groups: one infected with enterohemorrhagic E. coli (EHEC), another treated with date seed extract, and a control group. The extract was prepared using water immersion, followed by GC-MS analysis to identify active compounds. Major constituents included Trimethylolnitromethane, Glyceraldehyde, and 5-Hydroxymethylfurfural. Histological samples were collected from the colon, cecum, and rectum after 30 days of treatment. Real-time PCR was used to measure MUC1 and MUC4 gene expression.

Results:
The results showed that infected rats had downregulated MUC4 (~0.5 fold) and stable MUC1 expression, while healthy rats treated with date extract showed significant upregulation of MUC1 (~2.0 fold). Histopathological analysis confirmed inflammation, crypt damage, and lymphocytic infiltration in infected tissues, whereas treated tissues showed restored mucosal architecture and reduced inflammatory signs. ANOVA and Tukey’s post hoc tests indicated significant differences (P < 0.05) in gene expression among groups.

Conclusion:
The study concludes that date seed extract supports gastrointestinal health by modulating mucin gene expression and improving tissue recovery after infection. These findings suggest potential use of date seed supplements in managing gut inflammation and improving mucosal healing.

Key words: GC-MS; Gene expression; MUC-1; MUC4.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Maha Abdul-Hadi Abdul-Rida Al-Abdula
Articles by Saffia Kareem Wally Alumeri
Articles by Eman F. Albaghdady
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Al-abdula MAA, Alumeri SKW, Albaghdady EF. Molecular and histopathological investigation about the impact of Zuhdi Date palm (Phoenix dactylifera L.) seeds on infected and healthy female rats. Open Vet. J.. 2025; 15(11): 5523-5537. doi:10.5455/OVJ.2025.v15.i11.9

Web Style

Al-abdula MAA, Alumeri SKW, Albaghdady EF. Molecular and histopathological investigation about the impact of Zuhdi Date palm (Phoenix dactylifera L.) seeds on infected and healthy female rats. https://www.openveterinaryjournal.com/?mno=272456 [Access: November 30, 2025]. doi:10.5455/OVJ.2025.v15.i11.9

AMA (American Medical Association) Style

Al-abdula MAA, Alumeri SKW, Albaghdady EF. Molecular and histopathological investigation about the impact of Zuhdi Date palm (Phoenix dactylifera L.) seeds on infected and healthy female rats. Open Vet. J.. 2025; 15(11): 5523-5537. doi:10.5455/OVJ.2025.v15.i11.9


Vancouver/ICMJE Style

Al-abdula MAA, Alumeri SKW, Albaghdady EF. Molecular and histopathological investigation about the impact of Zuhdi Date palm (Phoenix dactylifera L.) seeds on infected and healthy female rats. Open Vet. J.. (2025), [cited November 30, 2025]; 15(11): 5523-5537. doi:10.5455/OVJ.2025.v15.i11.9


Harvard Style

Al-abdula, M. A. A., Alumeri, . S. K. W. & Albaghdady, . E. F. (2025) Molecular and histopathological investigation about the impact of Zuhdi Date palm (Phoenix dactylifera L.) seeds on infected and healthy female rats. Open Vet. J., 15 (11), 5523-5537. doi:10.5455/OVJ.2025.v15.i11.9


Turabian Style

Al-abdula, Maha Abdul-hadi Abdul-rida, Saffia Kareem Wally Alumeri, and Eman F. Albaghdady. 2025. Molecular and histopathological investigation about the impact of Zuhdi Date palm (Phoenix dactylifera L.) seeds on infected and healthy female rats. Open Veterinary Journal, 15 (11), 5523-5537. doi:10.5455/OVJ.2025.v15.i11.9


Chicago Style

Al-abdula, Maha Abdul-hadi Abdul-rida, Saffia Kareem Wally Alumeri, and Eman F. Albaghdady. "Molecular and histopathological investigation about the impact of Zuhdi Date palm (Phoenix dactylifera L.) seeds on infected and healthy female rats." Open Veterinary Journal 15 (2025), 5523-5537. doi:10.5455/OVJ.2025.v15.i11.9


MLA (The Modern Language Association) Style

Al-abdula, Maha Abdul-hadi Abdul-rida, Saffia Kareem Wally Alumeri, and Eman F. Albaghdady. "Molecular and histopathological investigation about the impact of Zuhdi Date palm (Phoenix dactylifera L.) seeds on infected and healthy female rats." Open Veterinary Journal 15.11 (2025), 5523-5537. Print. doi:10.5455/OVJ.2025.v15.i11.9


APA (American Psychological Association) Style

Al-abdula, M. A. A., Alumeri, . S. K. W. & Albaghdady, . E. F. (2025) Molecular and histopathological investigation about the impact of Zuhdi Date palm (Phoenix dactylifera L.) seeds on infected and healthy female rats. Open Veterinary Journal, 15 (11), 5523-5537. doi:10.5455/OVJ.2025.v15.i11.9


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense InformationCopyright Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldaghayes.com 

Email: ibrahim.eldaghayes@vetmed.edu.ly