E-ISSN 2218-6050 | ISSN 2226-4485
 
2026, Vol: 16, Issue: 5
16 / 5Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submissio Form
Title Page
mainarticle_template

Short Communication 


Effects of the methanolic extract of Tradescantia spathacea on nitro-oxidative and chemokine responses in peritoneal macrophages from BALB/c and C57BL/6 mice

Diana L. Chávez-aviña, Ángeles G. Lugo-díaz, Kibsaim Franco-villanueva, Víctor I. Lozano-vielmas, Gustavo Hernández-vidal, Raymundo A. Pérez-hernández, Adolfo Soto-dominguez, Uziel Castillo-velázquez.


Abstract
Background:
During innate immunity, peritoneal macrophages orchestrate early nitro-oxidative and chemokine responses, and their polarization is influenced by mouse strain–dependent metabolic programs. Tradescantia spathacea is a medicinal plant with reported anti-inflammatory and antioxidant properties; however, its effects on macrophage nitro-oxidative and chemokine responses remain unclear.

Aim:
To investigate the effects of a methanolic extract of T. spathacea (MET.s) on nitro-oxidative and chemokine responses in peritoneal macrophages (PMs) from BALB/c and C57BL/6 mice.

Methods:
LPS-elicited PMs were obtained from female BALB/c and C57BL/6 mice. The cytotoxicity and half-maximal inhibitory concentrations (IC50) of MET.s were determined by MTT assay. Nitric oxide (NO) production was quantified using the Griess reaction. Gene expression of iNOS, ARGII, TGF-β, MCP, and MIP was evaluated by real-time PCR in PMs stimulated with IL-4 or LPS, alone or in combination with strain-specific IC₅₀ concentrations of MET.s.

Results:
MET.s did not reduce mitochondrial activity in either strain; IC50 values were 4.76 µg/mL for BALB/c and 81.92 µg/mL for C57BL/6 PMs. BALB/c PMs exhibited a modest but significant increase in NO at 50 µg/mL, whereas C57BL/6 PMs exhibited decreased NO at the same concentration, inverting the expected strain pattern. In BALB/c PMs, MET.s alone or in combination with LPS or IL-4 favored iNOS and TGF-β expression, suggesting a pro-nitro-oxidative yet regulated profile. In C57BL/6 PMs, LPS+MET.s downregulated iNOS while upregulating ARGII and TGF-β, while MET.s selectively increased MIP with concurrent MCP reduction in both LPS- and IL-4-driven conditions, indicating a shift towards a balanced chemokine-mediated recruitment.

Conclusion:
The methanolic extract of T. spathacea exerts non-cytotoxic, strain-dependent immunomodulatory effects on peritoneal macrophages, reshaping nitro-oxidative output and chemokine gene expression to favor a context-dependent balance between activation and regulation. These findings support its potential as a natural immunomodulator and justify further mechanistic and in vivo studies.

Key words: BALB/c; C57BL/6; Chemokines; Peritoneal macrophages; Tradescantia spathacea.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Diana L. Chávez-Aviña
Articles by Ángeles G. Lugo-Díaz
Articles by Kibsaim Franco-Villanueva
Articles by Víctor I. Lozano-Vielmas
Articles by Gustavo Hernández-Vidal
Articles by Raymundo A. Pérez-Hernández
Articles by Adolfo Soto-Dominguez
Articles by Uziel Castillo-Velázquez
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Chávez-aviña DL, Lugo-díaz G, Franco-villanueva K, Lozano-vielmas VI, Hernández-vidal G, Pérez-hernández RA, Soto-dominguez A, Castillo-velázquez U. Effects of the methanolic extract of Tradescantia spathacea on nitro-oxidative and chemokine responses in peritoneal macrophages from BALB/c and C57BL/6 mice. doi:10.5455/OVJ.2026.v16.i5.63

Web Style

Chávez-aviña DL, Lugo-díaz G, Franco-villanueva K, Lozano-vielmas VI, Hernández-vidal G, Pérez-hernández RA, Soto-dominguez A, Castillo-velázquez U. Effects of the methanolic extract of Tradescantia spathacea on nitro-oxidative and chemokine responses in peritoneal macrophages from BALB/c and C57BL/6 mice. https://www.openveterinaryjournal.com/?mno=281775 [Access: May 31, 2026]. doi:10.5455/OVJ.2026.v16.i5.63

AMA (American Medical Association) Style

Chávez-aviña DL, Lugo-díaz G, Franco-villanueva K, Lozano-vielmas VI, Hernández-vidal G, Pérez-hernández RA, Soto-dominguez A, Castillo-velázquez U. Effects of the methanolic extract of Tradescantia spathacea on nitro-oxidative and chemokine responses in peritoneal macrophages from BALB/c and C57BL/6 mice. doi:10.5455/OVJ.2026.v16.i5.63


Vancouver/ICMJE Style

Chávez-aviña DL, Lugo-díaz G, Franco-villanueva K, Lozano-vielmas VI, Hernández-vidal G, Pérez-hernández RA, Soto-dominguez A, Castillo-velázquez U. Effects of the methanolic extract of Tradescantia spathacea on nitro-oxidative and chemokine responses in peritoneal macrophages from BALB/c and C57BL/6 mice. doi:10.5455/OVJ.2026.v16.i5.63


Harvard Style

Chávez-aviña, D. L., Lugo-díaz, . . G., Franco-villanueva, . K., Lozano-vielmas, . V. I., Hernández-vidal, . G., Pérez-hernández, . R. A., Soto-dominguez, . A. & Castillo-velázquez, . U. (2026) Effects of the methanolic extract of Tradescantia spathacea on nitro-oxidative and chemokine responses in peritoneal macrophages from BALB/c and C57BL/6 mice. doi:10.5455/OVJ.2026.v16.i5.63


Turabian Style

Chávez-aviña, Diana L., Ángeles G. Lugo-díaz, Kibsaim Franco-villanueva, Víctor I. Lozano-vielmas, Gustavo Hernández-vidal, Raymundo A. Pérez-hernández, Adolfo Soto-dominguez, and Uziel Castillo-velázquez. 2026. Effects of the methanolic extract of Tradescantia spathacea on nitro-oxidative and chemokine responses in peritoneal macrophages from BALB/c and C57BL/6 mice. doi:10.5455/OVJ.2026.v16.i5.63


Chicago Style

Chávez-aviña, Diana L., Ángeles G. Lugo-díaz, Kibsaim Franco-villanueva, Víctor I. Lozano-vielmas, Gustavo Hernández-vidal, Raymundo A. Pérez-hernández, Adolfo Soto-dominguez, and Uziel Castillo-velázquez. "Effects of the methanolic extract of Tradescantia spathacea on nitro-oxidative and chemokine responses in peritoneal macrophages from BALB/c and C57BL/6 mice." doi:10.5455/OVJ.2026.v16.i5.63


MLA (The Modern Language Association) Style

Chávez-aviña, Diana L., Ángeles G. Lugo-díaz, Kibsaim Franco-villanueva, Víctor I. Lozano-vielmas, Gustavo Hernández-vidal, Raymundo A. Pérez-hernández, Adolfo Soto-dominguez, and Uziel Castillo-velázquez. "Effects of the methanolic extract of Tradescantia spathacea on nitro-oxidative and chemokine responses in peritoneal macrophages from BALB/c and C57BL/6 mice." doi:10.5455/OVJ.2026.v16.i5.63


APA (American Psychological Association) Style

Chávez-aviña, D. L., Lugo-díaz, . . G., Franco-villanueva, . K., Lozano-vielmas, . V. I., Hernández-vidal, . G., Pérez-hernández, . R. A., Soto-dominguez, . A. & Castillo-velázquez, . U. (2026) Effects of the methanolic extract of Tradescantia spathacea on nitro-oxidative and chemokine responses in peritoneal macrophages from BALB/c and C57BL/6 mice. doi:10.5455/OVJ.2026.v16.i5.63


Author LoginReviewer LoginAbout PublisherOpen Access PolicyEditorial Review PolicyEditorial & Peer Review ProcessPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyProtection of Research Participants (Statement On Human And Animal Rights)Corrections, Retractions & Expressions of ConcernDigital Archiving & Preservation PoliciesTerms of UseLicense InformationCopyright Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Editor-in-Chief

Open Veterinary Journal

Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldaghayes.com 

Emails: ibrahim.eldaghayes@vetmed.edu.ly

info@eldaghayes.com