E-ISSN 2218-6050 | ISSN 2226-4485
 
2025, Vol: 15, Issue: 2
15 / 2Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles

Short Communication


Detection of virulence-associated genes in Brucella melitensis biovar 3, the prevalent field Strain in different animal species in Egypt

Mahmoud E R Hamdy, Hoda M Zaki.


Cited By:15

Abstract
The current study involved detection of three virulence genes (bvfA, virB, ure) by PCR in 52 isolates of Brucella melitensis biovar 3, recovered from different animal species (29 sheep, 10 goats, 9 cattle and 5 buffaloes). Of the 52 B. melitensis strains; 48 (92.3%) isolates carried bvfA genes, 51 (98.1%) isolates had virB genes and 50 (96.2%) isolates were positive for ure genes.
The distribution of the virulence genes is not affected by crossing the original host barriers of the animal species, as the three virulence factors (bvfA, virB and ure) detected in 29 B. melitensis isolates obtained from ovine species in a ratio of 27/29 (93.1%), 28/29 (96.6%) and 29/29 (100%), respectively. While 10 isolates originating from goats revealed a ratio of 10/10 (100%), 10/10 (100%) and 9/10 (90%) to the same order of virulence genes. Nearly, similar results of virulence genes detection were obtained in B. melitensis obtained from bovine (8/9, 9/9 and 8/9) and Buffalos (4/5, 5/5 and 5/5), respectively. The high prevalence of virulence-associated genes among the B. melitensis isolates detected from different animal species in Egypt is indicated a potential virulence of this bacterium.

Key words: Brucella melitensis biovar.3 – bvfA – virB - ure – PCR


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Mahmoud E R Hamdy
Articles by Hoda M Zaki
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Hamdy MER, Zaki HM. Detection of virulence-associated genes in Brucella melitensis biovar 3, the prevalent field Strain in different animal species in Egypt. Open Vet J. 2018; 8(1): 112-117. doi:10.4314/ovj.v8i1.17

Web Style

Hamdy MER, Zaki HM. Detection of virulence-associated genes in Brucella melitensis biovar 3, the prevalent field Strain in different animal species in Egypt. https://www.openveterinaryjournal.com/?mno=286052 [Access: March 19, 2025]. doi:10.4314/ovj.v8i1.17

AMA (American Medical Association) Style

Hamdy MER, Zaki HM. Detection of virulence-associated genes in Brucella melitensis biovar 3, the prevalent field Strain in different animal species in Egypt. Open Vet J. 2018; 8(1): 112-117. doi:10.4314/ovj.v8i1.17


Vancouver/ICMJE Style

Hamdy MER, Zaki HM. Detection of virulence-associated genes in Brucella melitensis biovar 3, the prevalent field Strain in different animal species in Egypt. Open Vet J. (2018), [cited March 19, 2025]; 8(1): 112-117. doi:10.4314/ovj.v8i1.17


Harvard Style

Hamdy, M. E. R. & Zaki, . H. M. (2018) Detection of virulence-associated genes in Brucella melitensis biovar 3, the prevalent field Strain in different animal species in Egypt. Open Vet J, 8 (1), 112-117. doi:10.4314/ovj.v8i1.17


Turabian Style

Hamdy, Mahmoud E R, and Hoda M Zaki. 2018. Detection of virulence-associated genes in Brucella melitensis biovar 3, the prevalent field Strain in different animal species in Egypt. Open Veterinary Journal, 8 (1), 112-117. doi:10.4314/ovj.v8i1.17


Chicago Style

Hamdy, Mahmoud E R, and Hoda M Zaki. "Detection of virulence-associated genes in Brucella melitensis biovar 3, the prevalent field Strain in different animal species in Egypt." Open Veterinary Journal 8 (2018), 112-117. doi:10.4314/ovj.v8i1.17


MLA (The Modern Language Association) Style

Hamdy, Mahmoud E R, and Hoda M Zaki. "Detection of virulence-associated genes in Brucella melitensis biovar 3, the prevalent field Strain in different animal species in Egypt." Open Veterinary Journal 8.1 (2018), 112-117. Print. doi:10.4314/ovj.v8i1.17


APA (American Psychological Association) Style

Hamdy, M. E. R. & Zaki, . H. M. (2018) Detection of virulence-associated genes in Brucella melitensis biovar 3, the prevalent field Strain in different animal species in Egypt. Open Veterinary Journal, 8 (1), 112-117. doi:10.4314/ovj.v8i1.17


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense InformationCopyright Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldagpublisher.com 

Email: ibrahim.eldaghayes@vetmed.edu.ly