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2025, Vol: 15, Issue: 2
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Effects of vitamin B12 supplementation on the quality of Ovine spermatozoa

M.a. Hamedani1, *, A.m. Tahmasbi1 And Y.j. Ahangari2.


Cited By:32

Abstract
The present study was undertaken to investigate the effect of various levels of vitamin B12 in Tris extender on semen quality of Dallagh rams following the cooling and freeze/thawing process. Semen was collected from six healthy and mature rams with an average body weight of 60.0 ± 5.0 Kg using an electro ejaculator. High quality samples were mixed and diluted in Tris extender supplemented with different concentrations of vitamin B12 (0, 1, 2 and 3 mg/ml). The semen aliquots were cooled and preserved at 5°C and their qualities were evaluated during pre-freezing and then the cooled semen samples were packaged into 0.25 ml straws. Straws were frozen in the vapor of liquid nitrogen, and were then stored at -196ºC. Straws were thawed seven days later and the characteristics of spermatozoa were examined. Results of this study showed that the effect of vitamin B12 on characteristics such as viability, motility, progressive motility and normality of spermatozoa were significant in pre and post freezing conditions (P<0.05). In conclusion, for long term storage of semen of Dallagh rams, we recommend using 2 mg/ml of vitamin B12 in semen extender.

Key words: Dallagh ram, Freezing, Semen characteristics, Vitamin B12.


 
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How to Cite this Article
Pubmed Style

Hamedani M, , Ahangari ATAY. Effects of vitamin B12 supplementation on the quality of Ovine spermatozoa. Open Vet J. 2013; 3(2): 140-144. doi:10.5455/OVJ.2013.v3.i2.p140

Web Style

Hamedani M, , Ahangari ATAY. Effects of vitamin B12 supplementation on the quality of Ovine spermatozoa. https://www.openveterinaryjournal.com/?mno=302656865 [Access: March 18, 2025]. doi:10.5455/OVJ.2013.v3.i2.p140

AMA (American Medical Association) Style

Hamedani M, , Ahangari ATAY. Effects of vitamin B12 supplementation on the quality of Ovine spermatozoa. Open Vet J. 2013; 3(2): 140-144. doi:10.5455/OVJ.2013.v3.i2.p140


Vancouver/ICMJE Style

Hamedani M, , Ahangari ATAY. Effects of vitamin B12 supplementation on the quality of Ovine spermatozoa. Open Vet J. (2013), [cited March 18, 2025]; 3(2): 140-144. doi:10.5455/OVJ.2013.v3.i2.p140


Harvard Style

Hamedani, M., , . & Ahangari, . A. T. A. Y. (2013) Effects of vitamin B12 supplementation on the quality of Ovine spermatozoa. Open Vet J, 3 (2), 140-144. doi:10.5455/OVJ.2013.v3.i2.p140


Turabian Style

Hamedani, M.a., , and A.m. Tahmasbi And Y.j. Ahangari. 2013. Effects of vitamin B12 supplementation on the quality of Ovine spermatozoa. Open Veterinary Journal, 3 (2), 140-144. doi:10.5455/OVJ.2013.v3.i2.p140


Chicago Style

Hamedani, M.a., , and A.m. Tahmasbi And Y.j. Ahangari. "Effects of vitamin B12 supplementation on the quality of Ovine spermatozoa." Open Veterinary Journal 3 (2013), 140-144. doi:10.5455/OVJ.2013.v3.i2.p140


MLA (The Modern Language Association) Style

Hamedani, M.a., , and A.m. Tahmasbi And Y.j. Ahangari. "Effects of vitamin B12 supplementation on the quality of Ovine spermatozoa." Open Veterinary Journal 3.2 (2013), 140-144. Print. doi:10.5455/OVJ.2013.v3.i2.p140


APA (American Psychological Association) Style

Hamedani, M., , . & Ahangari, . A. T. A. Y. (2013) Effects of vitamin B12 supplementation on the quality of Ovine spermatozoa. Open Veterinary Journal, 3 (2), 140-144. doi:10.5455/OVJ.2013.v3.i2.p140


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