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2026, Vol: 16, Issue: 5
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Research Article


Molecular diagnosis of methicillin-resistant Staphylococcus aureus in backyard chickens affected with bumble foot

Ezzalden B. Ghayib, Sumaya Y. A. Al-dabbagh.


Abstract
Background:
Staphylococcus aureus (S. aureus) is an important zoonotic infection that causes diseases in chickens.

Aim:
The study aimed to isolate of MRSA from chickens that suffered from bumblefoot and perform molecular diagnosis using the mecA gene and detection of protein A using the spa gene.

Methods:
The samples were subjected to standard bacteriological examinations, including morphological and cultural characteristics, biochemical properties, and antibiotic sensitivity tests. Polymerase chain reaction (PCR) was done for detection of S. aureus using the (nuc, mecA and spa) genes.

Results:
The study showed that S. aureus formed 68/100 (68%) from chickens with bumble foot disease, while only 49 / 68 (72%) of the isolates were resistant to methicillin according to the disc diffusion test. The results of molecular detection showed that all tested isolates possessed (nuc, mecA, and spa) genes that gave amplicon products at (166 bp,147 bp and 357 bp) respectively.

Conclusion:
The existence of MRSA in chicken samples comprises a threat to public health due to its potential transmission to humans and the environment.

Key words: Bumble foot; Chickens; Spa gene; MRSA.


 
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How to Cite this Article
Pubmed Style

Ghayib EB, Al-dabbagh SYA. Molecular diagnosis of methicillin-resistant Staphylococcus aureus in backyard chickens affected with bumble foot. Open Vet. J.. 2026; 16(5): 3060-3066. doi:10.5455/OVJ.2026.v16.i5.48

Web Style

Ghayib EB, Al-dabbagh SYA. Molecular diagnosis of methicillin-resistant Staphylococcus aureus in backyard chickens affected with bumble foot. https://www.openveterinaryjournal.com/?mno=304398 [Access: May 31, 2026]. doi:10.5455/OVJ.2026.v16.i5.48

AMA (American Medical Association) Style

Ghayib EB, Al-dabbagh SYA. Molecular diagnosis of methicillin-resistant Staphylococcus aureus in backyard chickens affected with bumble foot. Open Vet. J.. 2026; 16(5): 3060-3066. doi:10.5455/OVJ.2026.v16.i5.48


Vancouver/ICMJE Style

Ghayib EB, Al-dabbagh SYA. Molecular diagnosis of methicillin-resistant Staphylococcus aureus in backyard chickens affected with bumble foot. Open Vet. J.. (2026), [cited May 31, 2026]; 16(5): 3060-3066. doi:10.5455/OVJ.2026.v16.i5.48


Harvard Style

Ghayib, E. B. & Al-dabbagh, . S. Y. A. (2026) Molecular diagnosis of methicillin-resistant Staphylococcus aureus in backyard chickens affected with bumble foot. Open Vet. J., 16 (5), 3060-3066. doi:10.5455/OVJ.2026.v16.i5.48


Turabian Style

Ghayib, Ezzalden B., and Sumaya Y. A. Al-dabbagh. 2026. Molecular diagnosis of methicillin-resistant Staphylococcus aureus in backyard chickens affected with bumble foot. Open Veterinary Journal, 16 (5), 3060-3066. doi:10.5455/OVJ.2026.v16.i5.48


Chicago Style

Ghayib, Ezzalden B., and Sumaya Y. A. Al-dabbagh. "Molecular diagnosis of methicillin-resistant Staphylococcus aureus in backyard chickens affected with bumble foot." Open Veterinary Journal 16 (2026), 3060-3066. doi:10.5455/OVJ.2026.v16.i5.48


MLA (The Modern Language Association) Style

Ghayib, Ezzalden B., and Sumaya Y. A. Al-dabbagh. "Molecular diagnosis of methicillin-resistant Staphylococcus aureus in backyard chickens affected with bumble foot." Open Veterinary Journal 16.5 (2026), 3060-3066. Print. doi:10.5455/OVJ.2026.v16.i5.48


APA (American Psychological Association) Style

Ghayib, E. B. & Al-dabbagh, . S. Y. A. (2026) Molecular diagnosis of methicillin-resistant Staphylococcus aureus in backyard chickens affected with bumble foot. Open Veterinary Journal, 16 (5), 3060-3066. doi:10.5455/OVJ.2026.v16.i5.48


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