E-ISSN 2218-6050 | ISSN 2226-4485
 
2026, Vol: 16, Issue: 8
16 / 8Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submissio Form
Title Page
mainarticle_template

Research Article


Use of Galleria mellonella as an in vivo model to evaluate the efficacy of tocosh (Solanum tuberosum) against Campylobacter coli

Ashly Chagua, Paula Cristobal, Carlos Conte-junior, Miguel Rojas, César Lázaro Ashly Chagua, Paula Cristobal, Miguel Rojas, Carlos Conte, César Lázaro.


Abstract
Background:
Galleria mellonella larvae (GML) are an alternative model for evaluating the in vivo antimicrobial activity of several medicinal plants. Tocosh, a traditional Peruvian food made from fermented potatoes, has been shown to have in vitro antibacterial properties; however, its in vivo effects remain unknown. Antibiotic-resistant Campylobacter coli is a bacterium implicated in gastrointestinal diseases in humans.

Aim:
In this study, GML were used as an in vivo model to evaluate the efficacy of ethanolic extract of tocosh (EET) against C. coli.

Methods:
Three trials were conducted on GML: (i) Evaluation of EET (3.50, 2.63, 1.75, and 0.88 mg/larva) safety. (ii) Determination of LD50 with different C. coli concentrations (1 to 8 Log CFU/mL). (iii) Effect of the safety dosage of EET on GML infected with the LD50. In all trials, larval mortality was evaluated over 96 hours.

Results:
Dosage of 1.75 and 0.88 mg/larva of EET from both brands was harmless to GML. The LD50 was 7 Log CFU/mL for the SM19 strain and 6 Log CFU/mL for the ATCC strain. GML inoculated with the SM19 strain and treated with the EET (0.88 mg/larva) showed a survival rate greater than 70%, while those inoculated with the ATCC strain and treated with the EET (1.75 and 0.88 mg/larva) showed a survival rate greater than 75%.

Conclusion:
The results obtained support the use of GMLs as an alternative experimental model for studies of the efficacy of tocosh against C. coli, with potential applications in veterinary medicine, clinical, and food microbiology.

Key words: Antimicrobial activity; Campylobacter coli; Galleria mellonella; Medicinal plants; Tocosh.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Ashly Chagua
Articles by Paula Cristobal
Articles by Carlos Conte-junior
Articles by Miguel Rojas
Articles by César Lázaro Ashly Chagua
Articles by Paula Cristobal
Articles by Miguel Rojas
Articles by Carlos Conte
Articles by César Lázaro
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Chagua A, Cristobal P, Conte-junior C, Rojas M, Chagua CLA, Cristobal P, Rojas M, Conte C, Lázaro C. Use of Galleria mellonella as an in vivo model to evaluate the efficacy of tocosh (Solanum tuberosum) against Campylobacter coli. Open Vet. J.. 2026; 16(8): 5215-5229. doi:10.5455/OVJ.2026.v16.i8.17

Web Style

Chagua A, Cristobal P, Conte-junior C, Rojas M, Chagua CLA, Cristobal P, Rojas M, Conte C, Lázaro C. Use of Galleria mellonella as an in vivo model to evaluate the efficacy of tocosh (Solanum tuberosum) against Campylobacter coli. https://www.openveterinaryjournal.com/?mno=315439 [Access: August 08, 2026]. doi:10.5455/OVJ.2026.v16.i8.17

AMA (American Medical Association) Style

Chagua A, Cristobal P, Conte-junior C, Rojas M, Chagua CLA, Cristobal P, Rojas M, Conte C, Lázaro C. Use of Galleria mellonella as an in vivo model to evaluate the efficacy of tocosh (Solanum tuberosum) against Campylobacter coli. Open Vet. J.. 2026; 16(8): 5215-5229. doi:10.5455/OVJ.2026.v16.i8.17


Vancouver/ICMJE Style

Chagua A, Cristobal P, Conte-junior C, Rojas M, Chagua CLA, Cristobal P, Rojas M, Conte C, Lázaro C. Use of Galleria mellonella as an in vivo model to evaluate the efficacy of tocosh (Solanum tuberosum) against Campylobacter coli. Open Vet. J.. (2026), [cited August 08, 2026]; 16(8): 5215-5229. doi:10.5455/OVJ.2026.v16.i8.17


Harvard Style

Chagua, A., Cristobal, . P., Conte-junior, . C., Rojas, . M., Chagua, . C. L. A., Cristobal, . P., Rojas, . M., Conte, . C. & Lázaro, . C. (2026) Use of Galleria mellonella as an in vivo model to evaluate the efficacy of tocosh (Solanum tuberosum) against Campylobacter coli. Open Vet. J., 16 (8), 5215-5229. doi:10.5455/OVJ.2026.v16.i8.17


Turabian Style

Chagua, Ashly, Paula Cristobal, Carlos Conte-junior, Miguel Rojas, César Lázaro Ashly Chagua, Paula Cristobal, Miguel Rojas, Carlos Conte, and César Lázaro. 2026. Use of Galleria mellonella as an in vivo model to evaluate the efficacy of tocosh (Solanum tuberosum) against Campylobacter coli. Open Veterinary Journal, 16 (8), 5215-5229. doi:10.5455/OVJ.2026.v16.i8.17


Chicago Style

Chagua, Ashly, Paula Cristobal, Carlos Conte-junior, Miguel Rojas, César Lázaro Ashly Chagua, Paula Cristobal, Miguel Rojas, Carlos Conte, and César Lázaro. "Use of Galleria mellonella as an in vivo model to evaluate the efficacy of tocosh (Solanum tuberosum) against Campylobacter coli." Open Veterinary Journal 16 (2026), 5215-5229. doi:10.5455/OVJ.2026.v16.i8.17


MLA (The Modern Language Association) Style

Chagua, Ashly, Paula Cristobal, Carlos Conte-junior, Miguel Rojas, César Lázaro Ashly Chagua, Paula Cristobal, Miguel Rojas, Carlos Conte, and César Lázaro. "Use of Galleria mellonella as an in vivo model to evaluate the efficacy of tocosh (Solanum tuberosum) against Campylobacter coli." Open Veterinary Journal 16.8 (2026), 5215-5229. Print. doi:10.5455/OVJ.2026.v16.i8.17


APA (American Psychological Association) Style

Chagua, A., Cristobal, . P., Conte-junior, . C., Rojas, . M., Chagua, . C. L. A., Cristobal, . P., Rojas, . M., Conte, . C. & Lázaro, . C. (2026) Use of Galleria mellonella as an in vivo model to evaluate the efficacy of tocosh (Solanum tuberosum) against Campylobacter coli. Open Veterinary Journal, 16 (8), 5215-5229. doi:10.5455/OVJ.2026.v16.i8.17


Author LoginReviewer LoginAbout PublisherOpen Access PolicyEditorial PoliciesEditorial Review PolicyEditorial & Peer Review ProcessPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyProtection of Research Participants (Statement On Human And Animal Rights)Corrections, Retractions & Expressions of ConcernSelf-Archiving PoliciesDigital Archiving & Preservation PoliciesStatement of Informed ConsentAdvertising PolicyTerms of UseLicense InformationCopyright Information

About Open Veterinary Journal

Open Veterinary Journal provides high quality peer review process for original research articles, review articles, short communications and case reports i ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes

Editor-in-Chief

Open Veterinary Journal

Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

Email: ibrahim.eldaghayes@vetmed.edu.ly