E-ISSN 2218-6050 | ISSN 2226-4485
 
2024, Vol: 14, Issue: 11
14 / 11Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submission Form

Short Communication


Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR

Hannes Beims , Martina Janke, Werner von der Ohe , Michael Steinert.


Cited By:14

Abstract
American Foulbrood (AFB) is a devastating disease of honey bee (Apis meilifera) larvae caused by the spore-forming, Gram-positive bacterium Paenibacillus larvae. In most countries, the law requires mandatory reporting of AFB to the veterinary authority. To speed up detection and genotyping of P. larvae spores, we compared different culturing protocols on Columbia sheep blood agar (CSA) and developed a new multiplex qPCR to distinguish between the two relevant P. larvae genotypes ERIC I and ERIC II. As confirmed by P. larvae reference strains and field isolates, the new identification and genotyping protocol halves the time of current workflows, lessens labour-intension, allows a higher throughput of samples for monitoring, and permits a faster intervention to prevent the spread of AFB.

Key words: American Foulbrood, Paenibacillus larvae, rapid detection, multiplex quantitative PCR, ERIC genotyping


 
ARTICLE TOOLS
Abstract
PDF Fulltext
HTML Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Hannes Beims
Articles by Martina Janke
Articles by Werner von der Ohe
Articles by Michael Steinert
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

HB, Janke M, WvdO, Steinert M, . Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR. Open Vet J. 2020; 10(1): 53-58. doi:10.4314/ovj.v10i1.9

Web Style

HB, Janke M, WvdO, Steinert M, . Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR. https://www.openveterinaryjournal.com/?mno=49844 [Access: December 07, 2024]. doi:10.4314/ovj.v10i1.9

AMA (American Medical Association) Style

HB, Janke M, WvdO, Steinert M, . Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR. Open Vet J. 2020; 10(1): 53-58. doi:10.4314/ovj.v10i1.9


Vancouver/ICMJE Style

HB, Janke M, WvdO, Steinert M, . Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR. Open Vet J. (2020), [cited December 07, 2024]; 10(1): 53-58. doi:10.4314/ovj.v10i1.9


Harvard Style

, H. B., Janke, M., , W. v. d. O. ., Steinert, M. & (2020) Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR. Open Vet J, 10 (1), 53-58. doi:10.4314/ovj.v10i1.9


Turabian Style

, Hannes Beims, Martina Janke, Werner von der Ohe, Michael Steinert, and . 2020. Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR. Open Veterinary Journal, 10 (1), 53-58. doi:10.4314/ovj.v10i1.9


Chicago Style

, Hannes Beims, Martina Janke, Werner von der Ohe, Michael Steinert, and . "Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR." Open Veterinary Journal 10 (2020), 53-58. doi:10.4314/ovj.v10i1.9


MLA (The Modern Language Association) Style

, Hannes Beims, Martina Janke, Werner von der Ohe, Michael Steinert, and . "Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR." Open Veterinary Journal 10.1 (2020), 53-58. Print. doi:10.4314/ovj.v10i1.9


APA (American Psychological Association) Style

, H. B., Janke, M., , W. v. d. O. ., Steinert, M. & (2020) Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR. Open Veterinary Journal, 10 (1), 53-58. doi:10.4314/ovj.v10i1.9


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldagpublisher.com 

Email: ibrahim.eldaghayes@vetmed.edu.ly