E-ISSN 2218-6050 | ISSN 2226-4485
 

Original Article


Molecular genotyping, histopathological and immunohistochemical studies of bovine papillomatosis

Hasanain Gharban, Sattar Jabbar AL-Shaeli, Talal Jabal Hussen.


Cited By:6

Abstract
Background:
Bovine papillomatosis (BP) is considered the most common health problem in large cattle farms.
Aim:
This study attempts to confirm clinically suspected BP in cattle by PCR assay, histopathology, IHC, and genotyping analysis of local isolates.
Methods:
According to morphological appearance and lesion features, a cross sectional study of 54 clinically diagnosed BP cattle was assigned to this current investigation from May to August (2021) in Al-Kut district (Wasit Province, Iraq) private veterinary clinics using purposive sampling technique based on set criteria. The cattle were diagnosed clinically, and the tissues were collected and some fixed in 10% neutral buffered formalin and other stored frozen and examined by histopathological technique, immunohistochemistry, and PCR assays.
Results:
Using PCR assay, all cattle were positive for the BPV L1 gene. According to detect the L1 gene, analysis of the phylogenetic tree showed that local BPV cattle isolates were closely related to the NCBI-BLAST BPV type-1 and type-2 of the Polish equine isolate (KF284133.1) and BPV Brazilian Bos taurus isolate (MH187961.1), respectively. Histological detection showed there were acanthosis, hperkeratosis, epidermal thickening, severe infiltration of mononuclear cells, massive hemorrhage, dermal fibroplasia, multifocal spongiosis, moderate neovascularization, moderate to severe elongation of the retention ridge towards the dermis, parakeratosis, rings of calcification, and necrosis with nuclear pyknosis of some spinosum cells. Immunohistochemical findings of tumor necrosis factor-alpha (TNF-), epidermal growth factor receptor (EGFR) and Fascin showed a significant variation in values of immunoreaction in the dermis and epidermis. These results ranged from negative (0) to mild positive (+1) to moderate positive (+2) reactions.
Conclusion:
The study provided essential molecular and genotyping data to improve our knowledge by emphasizing the crucial of IHC as an elegant diagnostic method to detect cellular alterations.

Key words: Cattle warts, Iraq, Papillomavirus, PCR, Sequence


 
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How to Cite this Article
Pubmed Style

Gharban H, AL-Shaeli SJ, Hussen TJ. Molecular genotyping, histopathological and immunohistochemical studies of bovine papillomatosis. Open Vet J. 2023; 13(1): 26-41. doi:10.5455/OVJ.2023.v13.i1.4


Web Style

Gharban H, AL-Shaeli SJ, Hussen TJ. Molecular genotyping, histopathological and immunohistochemical studies of bovine papillomatosis. https://www.openveterinaryjournal.com/?mno=115559 [Access: February 13, 2024]. doi:10.5455/OVJ.2023.v13.i1.4


AMA (American Medical Association) Style

Gharban H, AL-Shaeli SJ, Hussen TJ. Molecular genotyping, histopathological and immunohistochemical studies of bovine papillomatosis. Open Vet J. 2023; 13(1): 26-41. doi:10.5455/OVJ.2023.v13.i1.4



Vancouver/ICMJE Style

Gharban H, AL-Shaeli SJ, Hussen TJ. Molecular genotyping, histopathological and immunohistochemical studies of bovine papillomatosis. Open Vet J. (2023), [cited February 13, 2024]; 13(1): 26-41. doi:10.5455/OVJ.2023.v13.i1.4



Harvard Style

Gharban, H., AL-Shaeli, . S. J. & Hussen, . T. J. (2023) Molecular genotyping, histopathological and immunohistochemical studies of bovine papillomatosis. Open Vet J, 13 (1), 26-41. doi:10.5455/OVJ.2023.v13.i1.4



Turabian Style

Gharban, Hasanain, Sattar Jabbar AL-Shaeli, and Talal Jabal Hussen. 2023. Molecular genotyping, histopathological and immunohistochemical studies of bovine papillomatosis. Open Veterinary Journal, 13 (1), 26-41. doi:10.5455/OVJ.2023.v13.i1.4



Chicago Style

Gharban, Hasanain, Sattar Jabbar AL-Shaeli, and Talal Jabal Hussen. "Molecular genotyping, histopathological and immunohistochemical studies of bovine papillomatosis." Open Veterinary Journal 13 (2023), 26-41. doi:10.5455/OVJ.2023.v13.i1.4



MLA (The Modern Language Association) Style

Gharban, Hasanain, Sattar Jabbar AL-Shaeli, and Talal Jabal Hussen. "Molecular genotyping, histopathological and immunohistochemical studies of bovine papillomatosis." Open Veterinary Journal 13.1 (2023), 26-41. Print. doi:10.5455/OVJ.2023.v13.i1.4



APA (American Psychological Association) Style

Gharban, H., AL-Shaeli, . S. J. & Hussen, . T. J. (2023) Molecular genotyping, histopathological and immunohistochemical studies of bovine papillomatosis. Open Veterinary Journal, 13 (1), 26-41. doi:10.5455/OVJ.2023.v13.i1.4