E-ISSN 2218-6050 | ISSN 2226-4485
 

Research Article


Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming

Sergio Morado, Ailen Aparicio, Daniela Pinchetti, Claudia Cecilia Arraztoa, Gabriel Alvarez, Cynthia Gutnisky, Deborah Neild, Gabriel Dalvit, Pablo Cetica.


Abstract
Background:
As the porcine oocyte is the most sensitive to low temperature damage, it has been difficult to cryopreserve compared to those from other domestic animals. However, at present, vitrification is used as a method for the cryopreservation of both oocytes and embryos in this species.
Aim:
Our aim was to analyze alterations in metabolic parameters in vitrified-warmed in vitro matured porcine oocytes at different post-warming recuperation times. Additionally, metaphase II plate recovery time analysis, in vitro fertilization and intracytoplasmic sperm injection were carried out to evaluate oocyte recovery capacity.
Methods:
Oocytes were vitrified-warmed and then incubated for 0, 3 or 21h post-warming to assess biochemical parameters.
Results:
Oocyte viability and morphology were not affected by vitrification-warming. Cytosolic oxidative status, active mitochondria and reactive oxygen species levels presented changes at the different time points in control and vitrified-warmed oocytes (P<0.05) as well as differences between both groups (P<0.05). NAD(P)H levels remained constant throughout different recuperation times, but were significantly lower in vitrified-warmed oocytes (P<0.05). Metaphase II plate recovery occurred mostly between 3 and 4h post-warming, but the percentage of metaphase II was reduced by vitrification-warming. Sperm head decondensation and pronuclear formation capacities were not modified.
Conclusion:
In conclusion, vitrification-warming generates biochemical alterations in porcine oocytes that would be, in part, responsible for affecting their performance. So, although the technique is a valid alternative for porcine oocyte cryopreservation, the protocols should be adapted to minimize those alterations.

Key words: Cryopreservation, Mitochondria, Porcine oocytes, Reactive oxygen species, Vitrification


 
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How to Cite this Article
Pubmed Style

Morado S, Aparicio A, Pinchetti D, Arraztoa CC, Alvarez G, Gutnisky C, Neild D, Dalvit G, Cetica P. Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming. Open Vet J. 2023; 13(11): 1416-1424. doi:10.5455/OVJ.2023.v13.i11.4


Web Style

Morado S, Aparicio A, Pinchetti D, Arraztoa CC, Alvarez G, Gutnisky C, Neild D, Dalvit G, Cetica P. Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming. https://www.openveterinaryjournal.com/?mno=160114 [Access: February 13, 2024]. doi:10.5455/OVJ.2023.v13.i11.4


AMA (American Medical Association) Style

Morado S, Aparicio A, Pinchetti D, Arraztoa CC, Alvarez G, Gutnisky C, Neild D, Dalvit G, Cetica P. Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming. Open Vet J. 2023; 13(11): 1416-1424. doi:10.5455/OVJ.2023.v13.i11.4



Vancouver/ICMJE Style

Morado S, Aparicio A, Pinchetti D, Arraztoa CC, Alvarez G, Gutnisky C, Neild D, Dalvit G, Cetica P. Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming. Open Vet J. (2023), [cited February 13, 2024]; 13(11): 1416-1424. doi:10.5455/OVJ.2023.v13.i11.4



Harvard Style

Morado, S., Aparicio, . A., Pinchetti, . D., Arraztoa, . C. C., Alvarez, . G., Gutnisky, . C., Neild, . D., Dalvit, . G. & Cetica, . P. (2023) Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming. Open Vet J, 13 (11), 1416-1424. doi:10.5455/OVJ.2023.v13.i11.4



Turabian Style

Morado, Sergio, Ailen Aparicio, Daniela Pinchetti, Claudia Cecilia Arraztoa, Gabriel Alvarez, Cynthia Gutnisky, Deborah Neild, Gabriel Dalvit, and Pablo Cetica. 2023. Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming. Open Veterinary Journal, 13 (11), 1416-1424. doi:10.5455/OVJ.2023.v13.i11.4



Chicago Style

Morado, Sergio, Ailen Aparicio, Daniela Pinchetti, Claudia Cecilia Arraztoa, Gabriel Alvarez, Cynthia Gutnisky, Deborah Neild, Gabriel Dalvit, and Pablo Cetica. "Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming." Open Veterinary Journal 13 (2023), 1416-1424. doi:10.5455/OVJ.2023.v13.i11.4



MLA (The Modern Language Association) Style

Morado, Sergio, Ailen Aparicio, Daniela Pinchetti, Claudia Cecilia Arraztoa, Gabriel Alvarez, Cynthia Gutnisky, Deborah Neild, Gabriel Dalvit, and Pablo Cetica. "Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming." Open Veterinary Journal 13.11 (2023), 1416-1424. Print. doi:10.5455/OVJ.2023.v13.i11.4



APA (American Psychological Association) Style

Morado, S., Aparicio, . A., Pinchetti, . D., Arraztoa, . C. C., Alvarez, . G., Gutnisky, . C., Neild, . D., Dalvit, . G. & Cetica, . P. (2023) Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming. Open Veterinary Journal, 13 (11), 1416-1424. doi:10.5455/OVJ.2023.v13.i11.4