E-ISSN 2218-6050 | ISSN 2226-4485
 
2024, Vol: 14, Issue: 10
14 / 10Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submission Form

Research Article


Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming

Sergio Morado, Ailen Aparicio, Daniela Pinchetti, Claudia Cecilia Arraztoa, Gabriel Alvarez, Cynthia Gutnisky, Deborah Neild, Gabriel Dalvit, Pablo Cetica.


Abstract
Background:
As the porcine oocyte is the most sensitive to low temperature damage, it has been difficult to cryopreserve compared to those from other domestic animals. However, at present, vitrification is used as a method for the cryopreservation of both oocytes and embryos in this species.
Aim:
Our aim was to analyze alterations in metabolic parameters in vitrified-warmed in vitro matured porcine oocytes at different post-warming recuperation times. Additionally, metaphase II plate recovery time analysis, in vitro fertilization and intracytoplasmic sperm injection were carried out to evaluate oocyte recovery capacity.
Methods:
Oocytes were vitrified-warmed and then incubated for 0, 3 or 21h post-warming to assess biochemical parameters.
Results:
Oocyte viability and morphology were not affected by vitrification-warming. Cytosolic oxidative status, active mitochondria and reactive oxygen species levels presented changes at the different time points in control and vitrified-warmed oocytes (P<0.05) as well as differences between both groups (P<0.05). NAD(P)H levels remained constant throughout different recuperation times, but were significantly lower in vitrified-warmed oocytes (P<0.05). Metaphase II plate recovery occurred mostly between 3 and 4h post-warming, but the percentage of metaphase II was reduced by vitrification-warming. Sperm head decondensation and pronuclear formation capacities were not modified.
Conclusion:
In conclusion, vitrification-warming generates biochemical alterations in porcine oocytes that would be, in part, responsible for affecting their performance. So, although the technique is a valid alternative for porcine oocyte cryopreservation, the protocols should be adapted to minimize those alterations.

Key words: Cryopreservation, Mitochondria, Porcine oocytes, Reactive oxygen species, Vitrification


 
ARTICLE TOOLS
Abstract
PDF Fulltext
HTML Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Sergio Morado
Articles by Ailen Aparicio
Articles by Daniela Pinchetti
Articles by Claudia Cecilia Arraztoa
Articles by Gabriel Alvarez
Articles by Cynthia Gutnisky
Articles by Deborah Neild
Articles by Gabriel Dalvit
Articles by Pablo Cetica
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Morado S, Aparicio A, Pinchetti D, Arraztoa CC, Alvarez G, Gutnisky C, Neild D, Dalvit G, Cetica P. Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming. Open Vet J. 2023; 13(11): 1416-1424. doi:10.5455/OVJ.2023.v13.i11.4

Web Style

Morado S, Aparicio A, Pinchetti D, Arraztoa CC, Alvarez G, Gutnisky C, Neild D, Dalvit G, Cetica P. Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming. https://www.openveterinaryjournal.com/?mno=160114 [Access: November 07, 2024]. doi:10.5455/OVJ.2023.v13.i11.4

AMA (American Medical Association) Style

Morado S, Aparicio A, Pinchetti D, Arraztoa CC, Alvarez G, Gutnisky C, Neild D, Dalvit G, Cetica P. Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming. Open Vet J. 2023; 13(11): 1416-1424. doi:10.5455/OVJ.2023.v13.i11.4


Vancouver/ICMJE Style

Morado S, Aparicio A, Pinchetti D, Arraztoa CC, Alvarez G, Gutnisky C, Neild D, Dalvit G, Cetica P. Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming. Open Vet J. (2023), [cited November 07, 2024]; 13(11): 1416-1424. doi:10.5455/OVJ.2023.v13.i11.4


Harvard Style

Morado, S., Aparicio, . A., Pinchetti, . D., Arraztoa, . C. C., Alvarez, . G., Gutnisky, . C., Neild, . D., Dalvit, . G. & Cetica, . P. (2023) Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming. Open Vet J, 13 (11), 1416-1424. doi:10.5455/OVJ.2023.v13.i11.4


Turabian Style

Morado, Sergio, Ailen Aparicio, Daniela Pinchetti, Claudia Cecilia Arraztoa, Gabriel Alvarez, Cynthia Gutnisky, Deborah Neild, Gabriel Dalvit, and Pablo Cetica. 2023. Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming. Open Veterinary Journal, 13 (11), 1416-1424. doi:10.5455/OVJ.2023.v13.i11.4


Chicago Style

Morado, Sergio, Ailen Aparicio, Daniela Pinchetti, Claudia Cecilia Arraztoa, Gabriel Alvarez, Cynthia Gutnisky, Deborah Neild, Gabriel Dalvit, and Pablo Cetica. "Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming." Open Veterinary Journal 13 (2023), 1416-1424. doi:10.5455/OVJ.2023.v13.i11.4


MLA (The Modern Language Association) Style

Morado, Sergio, Ailen Aparicio, Daniela Pinchetti, Claudia Cecilia Arraztoa, Gabriel Alvarez, Cynthia Gutnisky, Deborah Neild, Gabriel Dalvit, and Pablo Cetica. "Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming." Open Veterinary Journal 13.11 (2023), 1416-1424. Print. doi:10.5455/OVJ.2023.v13.i11.4


APA (American Psychological Association) Style

Morado, S., Aparicio, . A., Pinchetti, . D., Arraztoa, . C. C., Alvarez, . G., Gutnisky, . C., Neild, . D., Dalvit, . G. & Cetica, . P. (2023) Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming. Open Veterinary Journal, 13 (11), 1416-1424. doi:10.5455/OVJ.2023.v13.i11.4


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldagpublisher.com 

Email: ibrahim.eldaghayes@vetmed.edu.ly