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2025, Vol: 15, Issue: 2
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Research Article


Bacteriological evaluation and advanced SYBR-green multiplex real-time PCR assay for detection of minced meat adulteration

Soad H. El-sheikh, Reham M. Abdel Whab, Rania A. Eldaly, Mona T. Raslan, Hanan A. Fahmy, Azza S. El-demerdash.


Cited By:1

Abstract
Background:
Minced meat is a valuable source of nutrients, but it is vulnerable to contamination by microorganisms commonly present in the environment. Additionally, there is a risk of adulteration with cheaper meat sources, which can be harmful to consumers.
Aim:
It is crucial to identify meat adulteration with distinct microbiological analysis for legal, economic, religious, and public health purposes.
Methods:
A total of 100 minced meat samples were collected from several markets in Sharkia Governorate, Egypt. These samples were then subjected to bacteriological testing and an advanced multiplex PCR method. This method enables the detection of bovine, equine, porcine, and dog species in meat samples with just one step.
Results:
The adulterated samples had a higher total bacterial count and pH values compared to pure bovine meat. These differences in bacterial count and pH values were statistically significant, with p-values of 0.843 (log10) and 0.233, respectively. The frequency of E. coli occurrence was 13%, and the O111 serotype was predominant in the adulterated samples. Listeria monocytogenes and S. aureus were isolated with prevalence rates of 3% and 29%, respectively. Besides, the SYBR-green multiplex real-time PCR assay used in this study detected adulteration with dog, equine, and porcine meats in the examined samples at rates of 9%, 5%, and 4%, respectively.
Conclusion:
This method provides a sensitive and specific approach to detect issues related to well-being and safety.

Key words: Bacteriological examination, Meat adulteration, Multiplex PCR assay, PH values


 
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How to Cite this Article
Pubmed Style

El-sheikh SH, Whab RMA, Eldaly RA, Raslan MT, Fahmy HA, El-demerdash AS. Bacteriological evaluation and advanced SYBR-green multiplex real-time PCR assay for detection of minced meat adulteration. Open Vet J. 2024; 14((1) (Zagazig Veterinary Conference)): 389-397. doi:10.5455/OVJ.2024.v14.i1.35

Web Style

El-sheikh SH, Whab RMA, Eldaly RA, Raslan MT, Fahmy HA, El-demerdash AS. Bacteriological evaluation and advanced SYBR-green multiplex real-time PCR assay for detection of minced meat adulteration. https://www.openveterinaryjournal.com/?mno=178806 [Access: March 18, 2025]. doi:10.5455/OVJ.2024.v14.i1.35

AMA (American Medical Association) Style

El-sheikh SH, Whab RMA, Eldaly RA, Raslan MT, Fahmy HA, El-demerdash AS. Bacteriological evaluation and advanced SYBR-green multiplex real-time PCR assay for detection of minced meat adulteration. Open Vet J. 2024; 14((1) (Zagazig Veterinary Conference)): 389-397. doi:10.5455/OVJ.2024.v14.i1.35


Vancouver/ICMJE Style

El-sheikh SH, Whab RMA, Eldaly RA, Raslan MT, Fahmy HA, El-demerdash AS. Bacteriological evaluation and advanced SYBR-green multiplex real-time PCR assay for detection of minced meat adulteration. Open Vet J. (2024), [cited March 18, 2025]; 14((1) (Zagazig Veterinary Conference)): 389-397. doi:10.5455/OVJ.2024.v14.i1.35


Harvard Style

El-sheikh, S. H., Whab, . R. M. A., Eldaly, . R. A., Raslan, . M. T., Fahmy, . H. A. & El-demerdash, . A. S. (2024) Bacteriological evaluation and advanced SYBR-green multiplex real-time PCR assay for detection of minced meat adulteration. Open Vet J, 14 ((1) (Zagazig Veterinary Conference)), 389-397. doi:10.5455/OVJ.2024.v14.i1.35


Turabian Style

El-sheikh, Soad H., Reham M. Abdel Whab, Rania A. Eldaly, Mona T. Raslan, Hanan A. Fahmy, and Azza S. El-demerdash. 2024. Bacteriological evaluation and advanced SYBR-green multiplex real-time PCR assay for detection of minced meat adulteration. Open Veterinary Journal, 14 ((1) (Zagazig Veterinary Conference)), 389-397. doi:10.5455/OVJ.2024.v14.i1.35


Chicago Style

El-sheikh, Soad H., Reham M. Abdel Whab, Rania A. Eldaly, Mona T. Raslan, Hanan A. Fahmy, and Azza S. El-demerdash. "Bacteriological evaluation and advanced SYBR-green multiplex real-time PCR assay for detection of minced meat adulteration." Open Veterinary Journal 14 (2024), 389-397. doi:10.5455/OVJ.2024.v14.i1.35


MLA (The Modern Language Association) Style

El-sheikh, Soad H., Reham M. Abdel Whab, Rania A. Eldaly, Mona T. Raslan, Hanan A. Fahmy, and Azza S. El-demerdash. "Bacteriological evaluation and advanced SYBR-green multiplex real-time PCR assay for detection of minced meat adulteration." Open Veterinary Journal 14.(1) (Zagazig Veterinary Conference) (2024), 389-397. Print. doi:10.5455/OVJ.2024.v14.i1.35


APA (American Psychological Association) Style

El-sheikh, S. H., Whab, . R. M. A., Eldaly, . R. A., Raslan, . M. T., Fahmy, . H. A. & El-demerdash, . A. S. (2024) Bacteriological evaluation and advanced SYBR-green multiplex real-time PCR assay for detection of minced meat adulteration. Open Veterinary Journal, 14 ((1) (Zagazig Veterinary Conference)), 389-397. doi:10.5455/OVJ.2024.v14.i1.35


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