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Research Article


Effect of two different commercial vaccines against bovine respiratory disease on cell mediated immunity in Holstein cattle

Mostafa El-Sayed El-Sheikh, Mamdouh Fahmy El-Mekawy, Mohammed Ibrahim Eisa, Nasser Zeidan Abouzeid, Mervat Ibrahim Abdelmonim, Emad Mohamed Bennour, Sarah Gamal Yousef.


Abstract
Background:
Bovine respiratory disease (BRD) is a complex illness that impacts the respiratory system of domestic cattle, resulting in significant financial losses for the agriculture industry. Inactivated or modified live pathogen vaccines are often used as a management tool to prevent and control BRD effectively.
Aim:
The purpose of this study is to assess the cell-mediated immune response (CMI) induced by two commercially available polyvalent vaccines, namely the modified live (Cattle Master Gold FP) and the inactivated (CATTLEWIN-5K) vaccine.
Methods:
A total of 20 seronegative heifers against 4 BRD viruses, Bovine alphaherpisvirus-1 (BoAHV-1), Bovine viral diarrhea virus (BVDV BVDV-1: Pesti virus A; BVDV-2: Pesti virus B), bovine respiratory syncytial virus (BRSV) and Bovine parainfluenza virus-3 (BPIV3) were chosen for this study. The heifers were divided into three groups. The first group (n=6) received no vaccination and was kept as a control. The second and third groups (7 heifers each) were vaccinated twice with either a modified live (MLV) or inactivated vaccine. The gene expression level of interleukin-6 (IL-6) and interferon-gamma (INF-γ) was measured using real-time quantitative PCR (RT-qPCR) on the 7th, 14th, 21st, 28th, and 60th days post-vaccination. The results were compared with the control group to study the effectiveness of the vaccines.
Results:
There was an upregulation in the expression level of IL-6 and INF-γ in both MLV and inactivated vaccinated groups. The level of IL-6 mRNA expression was statistically increased from the 14th and 28th days post-vaccination in MLV and inactivated vaccine groups, respectively. The expression level of INF-γ increased significantly from the 2nd and 4th weeks post-vaccination in the MLV and inactivated vaccine groups, respectively. The mean expression level of IL-6 and INF-γ mRNAs was significantly higher in the MLV vaccine group than in the inactivated vaccine group at each examination time.
Conclusion:
Both investigated vaccines are efficient in stimulating CMI, particularly with the MLV vaccine showing a higher preponderance in IL-6 and INF-γ.

Key words: BRD, Cell mediated immunity, IL-6, INF-γ, Vaccine


 
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How to Cite this Article
Pubmed Style

El-Sheikh ME, El-Mekawy MF, Eisa MI, Abouzeid NZ, Abdelmonim MI, Bennour EM, Yousef SG. Effect of two different commercial vaccines against bovine respiratory disease on cell mediated immunity in Holstein cattle. Open Vet J. 2024; 14(8): 1921-1927. doi:10.5455/OVJ.2024.v14.i8.20

Web Style

El-Sheikh ME, El-Mekawy MF, Eisa MI, Abouzeid NZ, Abdelmonim MI, Bennour EM, Yousef SG. Effect of two different commercial vaccines against bovine respiratory disease on cell mediated immunity in Holstein cattle. https://www.openveterinaryjournal.com/?mno=200501 [Access: September 16, 2024]. doi:10.5455/OVJ.2024.v14.i8.20

AMA (American Medical Association) Style

El-Sheikh ME, El-Mekawy MF, Eisa MI, Abouzeid NZ, Abdelmonim MI, Bennour EM, Yousef SG. Effect of two different commercial vaccines against bovine respiratory disease on cell mediated immunity in Holstein cattle. Open Vet J. 2024; 14(8): 1921-1927. doi:10.5455/OVJ.2024.v14.i8.20


Vancouver/ICMJE Style

El-Sheikh ME, El-Mekawy MF, Eisa MI, Abouzeid NZ, Abdelmonim MI, Bennour EM, Yousef SG. Effect of two different commercial vaccines against bovine respiratory disease on cell mediated immunity in Holstein cattle. Open Vet J. (2024), [cited September 16, 2024]; 14(8): 1921-1927. doi:10.5455/OVJ.2024.v14.i8.20


Harvard Style

El-Sheikh, M. E., El-Mekawy, . M. F., Eisa, . M. I., Abouzeid, . N. Z., Abdelmonim, . M. I., Bennour, . E. M. & Yousef, . S. G. (2024) Effect of two different commercial vaccines against bovine respiratory disease on cell mediated immunity in Holstein cattle. Open Vet J, 14 (8), 1921-1927. doi:10.5455/OVJ.2024.v14.i8.20


Turabian Style

El-Sheikh, Mostafa El-Sayed, Mamdouh Fahmy El-Mekawy, Mohammed Ibrahim Eisa, Nasser Zeidan Abouzeid, Mervat Ibrahim Abdelmonim, Emad Mohamed Bennour, and Sarah Gamal Yousef. 2024. Effect of two different commercial vaccines against bovine respiratory disease on cell mediated immunity in Holstein cattle. Open Veterinary Journal, 14 (8), 1921-1927. doi:10.5455/OVJ.2024.v14.i8.20


Chicago Style

El-Sheikh, Mostafa El-Sayed, Mamdouh Fahmy El-Mekawy, Mohammed Ibrahim Eisa, Nasser Zeidan Abouzeid, Mervat Ibrahim Abdelmonim, Emad Mohamed Bennour, and Sarah Gamal Yousef. "Effect of two different commercial vaccines against bovine respiratory disease on cell mediated immunity in Holstein cattle." Open Veterinary Journal 14 (2024), 1921-1927. doi:10.5455/OVJ.2024.v14.i8.20


MLA (The Modern Language Association) Style

El-Sheikh, Mostafa El-Sayed, Mamdouh Fahmy El-Mekawy, Mohammed Ibrahim Eisa, Nasser Zeidan Abouzeid, Mervat Ibrahim Abdelmonim, Emad Mohamed Bennour, and Sarah Gamal Yousef. "Effect of two different commercial vaccines against bovine respiratory disease on cell mediated immunity in Holstein cattle." Open Veterinary Journal 14.8 (2024), 1921-1927. Print. doi:10.5455/OVJ.2024.v14.i8.20


APA (American Psychological Association) Style

El-Sheikh, M. E., El-Mekawy, . M. F., Eisa, . M. I., Abouzeid, . N. Z., Abdelmonim, . M. I., Bennour, . E. M. & Yousef, . S. G. (2024) Effect of two different commercial vaccines against bovine respiratory disease on cell mediated immunity in Holstein cattle. Open Veterinary Journal, 14 (8), 1921-1927. doi:10.5455/OVJ.2024.v14.i8.20


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