E-ISSN 2218-6050 | ISSN 2226-4485
 

Original Article


The effect of cryopreservation media on the quality of β-thalassemia mouse spermatozoa

Kakanang Buranaamnuay.


Cited By:2

Abstract
Background: The mouse model of human diseases is commonly used for biomedical study, including β-thalassemia (β-thal), an inherited hemoglobin disorder. Maintaining the mice strain by natural mating systems is costly and seems impractical, especially during the COVID-19 pandemic. Sperm freezing is a cost-effective solution for β-thal mouse colony management.
Aim: The present study was conducted to determine appropriate cryopreservation media for β-thal mouse spermatozoa to establish a β-thal mouse sperm bank.
Methods: The epididymal spermatozoa of C57BL/6 wild-type (WT) and -globin gene knockout thalassemia (BKO) mice were frozen in four freezing media: I) raffinose-skim milk-monothioglycerol (MTG), II) raffinose-skim milk-glutamine, III) raffinose-egg yolk-glycerol, and IV) egg yolk-TES-Tris. The sperm quality was assessed prior to and following freeze-thawing. Results: Compared with WT counterparts, the viable spermatozoa before freezing exhibiting elevated levels of oxidative stress were significantly greater in BKO (p = 0.01). After thawing, the membrane integrity of BKO spermatozoa preserved in I was significantly lower (p = 0.001). The sperm viability and membrane integrity of BKO males were also inferior when media III and IV were used (p = 0.008 to p = 0.027). The amount of oxidative stress in the spermatozoon of BKO mice was significantly greater when preserved in I, III, and IV (p = 0.002 to p = 0.044). Comparing freezing media, the motility and acrosome integrity of WT and BKO spermatozoa preserved in IV were significantly higher than those in other media (p < 0.001 to p = 0.01). Spermatozoa with the highest mitochondrial membrane potential (MMP) were observed in I in both genotypes (p = 0.012 to p > 0.05). The viability, membrane integrity, and oxidative stress of post-thaw BKO spermatozoa did not significantly differ among freezing solutions.
Conclusion: Irrespective of freezing media, spermatozoa of BKO males are rather more sensitive to cryopreservation than those of WT. Raffinose-skim milk-MTG/glutamine, raffinose-egg yolk-glycerol, and egg yolk-TES-Tris can all be used to preserve BKO mouse spermatozoa. However, with slightly better sperm characteristics, egg yolk-TES-Tris may be a diluent of choice for BKO mouse sperm cryopreservation. The addition of a reducing agent to thawing media is also strongly recommended to efficiently prevent oxidative stress and therefore improve frozen-thawed sperm survival.

Key words: Anemia, Freezing extender, Mouse, Sperm characteristics


 
ARTICLE TOOLS
Abstract
PDF Fulltext
HTML Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Kakanang Buranaamnuay
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Buranaamnuay K, . The effect of cryopreservation media on the quality of β-thalassemia mouse spermatozoa. Open Vet J. 2022; 12(5): 602-611. doi:10.5455/OVJ.2022.v12.i5.2


Web Style

Buranaamnuay K, . The effect of cryopreservation media on the quality of β-thalassemia mouse spermatozoa. https://www.openveterinaryjournal.com/?mno=20388 [Access: December 07, 2024]. doi:10.5455/OVJ.2022.v12.i5.2


AMA (American Medical Association) Style

Buranaamnuay K, . The effect of cryopreservation media on the quality of β-thalassemia mouse spermatozoa. Open Vet J. 2022; 12(5): 602-611. doi:10.5455/OVJ.2022.v12.i5.2



Vancouver/ICMJE Style

Buranaamnuay K, . The effect of cryopreservation media on the quality of β-thalassemia mouse spermatozoa. Open Vet J. (2022), [cited December 07, 2024]; 12(5): 602-611. doi:10.5455/OVJ.2022.v12.i5.2



Harvard Style

Buranaamnuay, K. & (2022) The effect of cryopreservation media on the quality of β-thalassemia mouse spermatozoa. Open Vet J, 12 (5), 602-611. doi:10.5455/OVJ.2022.v12.i5.2



Turabian Style

Buranaamnuay, Kakanang, and . 2022. The effect of cryopreservation media on the quality of β-thalassemia mouse spermatozoa. Open Veterinary Journal, 12 (5), 602-611. doi:10.5455/OVJ.2022.v12.i5.2



Chicago Style

Buranaamnuay, Kakanang, and . "The effect of cryopreservation media on the quality of β-thalassemia mouse spermatozoa." Open Veterinary Journal 12 (2022), 602-611. doi:10.5455/OVJ.2022.v12.i5.2



MLA (The Modern Language Association) Style

Buranaamnuay, Kakanang, and . "The effect of cryopreservation media on the quality of β-thalassemia mouse spermatozoa." Open Veterinary Journal 12.5 (2022), 602-611. Print. doi:10.5455/OVJ.2022.v12.i5.2



APA (American Psychological Association) Style

Buranaamnuay, K. & (2022) The effect of cryopreservation media on the quality of β-thalassemia mouse spermatozoa. Open Veterinary Journal, 12 (5), 602-611. doi:10.5455/OVJ.2022.v12.i5.2