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2025, Vol: 15, Issue: 2
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Research Article


Frozen semen quality of Pasundan bulls with different individual variations

Abdullah Baharun, Pirda Parida Permadani Pertiwi, Annisa Rahmi, Natashya Chandra Rachmadanti, Ristika Handarini, Hikmayani Iskandar, Daud Samsudewa, Tulus Maulana, Syahruddin Said, Imam Darussalam, Nurcholis Nurcholis, Raden Iis Arifiantini.


Abstract
Background:
Pasundan bulls are one of Indonesia's indigenous cattle breeds. The quality of frozen semen is essential for the success of artificial insemination (AI), as individual variations in parameters such as sperm motility, viability, and membrane integrity greatly influence reproductive outcomes.

Aim:
The objective of this study was to evaluate the quality of frozen semen from Pasundan bulls with different individual variations.

Methods:
Frozen semen from eight Pasundan bulls were obtained from the West Java Regional Artificial Insemination Center (RAIC). Sperm motility was assessed using computer-assisted sperm analysis (CASA; SpermVision®, Germany). Sperm viability and abnormalities were determined by eosin-nigrosine staining. Plasma membrane integrity was evaluated using the hypoosmotic swelling (HOS) test, acrosome integrity was assessed using fluorescein isothiocyanate-propidium iodide (FITC-PNA-PI), and protamine deficiency was measured using chromomycin A3 fluorescence staining.

Results:
Significant individual variations (p < 0.05) were found across all parameters of frozen semen quality, except for sperm abnormalities, which showed no significant differences (p > 0.05).

Conclusion:
The results indicate that individual variations significantly impact all quality parameters of frozen semen from Pasundan bulls, with the exception of sperm abnormalities. A comprehensive evaluation of individual bulls is therefore essential for producing high-quality frozen semen.

Key words: Fluorescent, Local bulls, Comprehensive evaluation, Pasundan bull


 
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How to Cite this Article
Pubmed Style

Baharun A, Pertiwi PPP, Rahmi A, Rachmadanti NC, Handarini R, Iskandar H, Samsudewa D, Maulana T, Said S, Darussalam I, Nurcholis N, Arifiantini RI. Frozen semen quality of Pasundan bulls with different individual variations. Open Vet J. 2025; 15(1): 244-251. doi:10.5455/OVJ.2025.v15.i1.22

Web Style

Baharun A, Pertiwi PPP, Rahmi A, Rachmadanti NC, Handarini R, Iskandar H, Samsudewa D, Maulana T, Said S, Darussalam I, Nurcholis N, Arifiantini RI. Frozen semen quality of Pasundan bulls with different individual variations. https://www.openveterinaryjournal.com/?mno=219914 [Access: March 19, 2025]. doi:10.5455/OVJ.2025.v15.i1.22

AMA (American Medical Association) Style

Baharun A, Pertiwi PPP, Rahmi A, Rachmadanti NC, Handarini R, Iskandar H, Samsudewa D, Maulana T, Said S, Darussalam I, Nurcholis N, Arifiantini RI. Frozen semen quality of Pasundan bulls with different individual variations. Open Vet J. 2025; 15(1): 244-251. doi:10.5455/OVJ.2025.v15.i1.22


Vancouver/ICMJE Style

Baharun A, Pertiwi PPP, Rahmi A, Rachmadanti NC, Handarini R, Iskandar H, Samsudewa D, Maulana T, Said S, Darussalam I, Nurcholis N, Arifiantini RI. Frozen semen quality of Pasundan bulls with different individual variations. Open Vet J. (2025), [cited March 19, 2025]; 15(1): 244-251. doi:10.5455/OVJ.2025.v15.i1.22


Harvard Style

Baharun, A., Pertiwi, . P. P. P., Rahmi, . A., Rachmadanti, . N. C., Handarini, . R., Iskandar, . H., Samsudewa, . D., Maulana, . T., Said, . S., Darussalam, . I., Nurcholis, . N. & Arifiantini, . R. I. (2025) Frozen semen quality of Pasundan bulls with different individual variations. Open Vet J, 15 (1), 244-251. doi:10.5455/OVJ.2025.v15.i1.22


Turabian Style

Baharun, Abdullah, Pirda Parida Permadani Pertiwi, Annisa Rahmi, Natashya Chandra Rachmadanti, Ristika Handarini, Hikmayani Iskandar, Daud Samsudewa, Tulus Maulana, Syahruddin Said, Imam Darussalam, Nurcholis Nurcholis, and Raden Iis Arifiantini. 2025. Frozen semen quality of Pasundan bulls with different individual variations. Open Veterinary Journal, 15 (1), 244-251. doi:10.5455/OVJ.2025.v15.i1.22


Chicago Style

Baharun, Abdullah, Pirda Parida Permadani Pertiwi, Annisa Rahmi, Natashya Chandra Rachmadanti, Ristika Handarini, Hikmayani Iskandar, Daud Samsudewa, Tulus Maulana, Syahruddin Said, Imam Darussalam, Nurcholis Nurcholis, and Raden Iis Arifiantini. "Frozen semen quality of Pasundan bulls with different individual variations." Open Veterinary Journal 15 (2025), 244-251. doi:10.5455/OVJ.2025.v15.i1.22


MLA (The Modern Language Association) Style

Baharun, Abdullah, Pirda Parida Permadani Pertiwi, Annisa Rahmi, Natashya Chandra Rachmadanti, Ristika Handarini, Hikmayani Iskandar, Daud Samsudewa, Tulus Maulana, Syahruddin Said, Imam Darussalam, Nurcholis Nurcholis, and Raden Iis Arifiantini. "Frozen semen quality of Pasundan bulls with different individual variations." Open Veterinary Journal 15.1 (2025), 244-251. Print. doi:10.5455/OVJ.2025.v15.i1.22


APA (American Psychological Association) Style

Baharun, A., Pertiwi, . P. P. P., Rahmi, . A., Rachmadanti, . N. C., Handarini, . R., Iskandar, . H., Samsudewa, . D., Maulana, . T., Said, . S., Darussalam, . I., Nurcholis, . N. & Arifiantini, . R. I. (2025) Frozen semen quality of Pasundan bulls with different individual variations. Open Veterinary Journal, 15 (1), 244-251. doi:10.5455/OVJ.2025.v15.i1.22


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