E-ISSN 2218-6050 | ISSN 2226-4485
 

Original Article


Expression of various sarcomeric tropomyosin isoforms in equine striated muscles

Syamalima Dube, Henry Chionuma, Amr Matoq, Ruham Alshiekh-Nasany, Lynn Abbott, Bernard J. Poiesz, Dipak K. Dube.


Cited By:7

Abstract
In order to better understand the training and athletic activity of horses, we must have complete understanding of the isoform diversity of various myofibrillar protein genes like tropomyosin. Tropomyosin (TPM), a coiled-coil dimeric protein, is a component of thin filament in striated muscles. In mammals, four TPM genes (TPM1, TPM2, TPM3, and TPM4) generate a multitude of TPM isoforms via alternate splicing and/or using different promoters. Unfortunately, our knowledge of TPM isoform diversity in the horse is very limited. Hence, we undertook a comprehensive exploratory study of various TPM isoforms from horse heart and skeletal muscle. We have cloned and sequenced two sarcomeric isoforms of the TPM1 gene called TPM1α and TPM1κ, one sarcomeric isoform of the TPM2 and one of the TPM3 gene, TPM2α and TPM3α respectively. By qRT-PCR using both relative expression and copy number, we have shown that TPM1α expression compared to TPM1κ is very high in heart. On the other hand, the expression of TPM1α is higher in skeletal muscle compared to heart. Further, the expression of TPM2α and TPM3α are higher in skeletal muscle compared to heart. Using western blot analyses with CH1 monoclonal antibody we have shown the high expression levels of sarcomeric TPM proteins in cardiac and skeletal muscle. Due to the paucity of isoform specific antibodies we cannot specifically detect the expression of TPM1κ in horse striated muscle. To the best of our knowledge this is the very first report on the characterization of sarcmeric TPMs in horse striated muscle.

Key words: horse, TPM, RT-PCR, qRT-PCR, relative expression, absolute copy number


 
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How to Cite this Article
Pubmed Style

Syamalima Dube, Henry Chionuma, Amr Matoq, Ruham Alshiekh-Nasany, Lynn Abbott, Bernard J. Poiesz, Dipak K. Dube. Expression of various sarcomeric tropomyosin isoforms in equine striated muscles. Open Vet J. 2017; 7(2): 180-191. doi:10.4314/ovj.v7i2.17


Web Style

Syamalima Dube, Henry Chionuma, Amr Matoq, Ruham Alshiekh-Nasany, Lynn Abbott, Bernard J. Poiesz, Dipak K. Dube. Expression of various sarcomeric tropomyosin isoforms in equine striated muscles. https://www.openveterinaryjournal.com/?mno=257235 [Access: February 28, 2024]. doi:10.4314/ovj.v7i2.17


AMA (American Medical Association) Style

Syamalima Dube, Henry Chionuma, Amr Matoq, Ruham Alshiekh-Nasany, Lynn Abbott, Bernard J. Poiesz, Dipak K. Dube. Expression of various sarcomeric tropomyosin isoforms in equine striated muscles. Open Vet J. 2017; 7(2): 180-191. doi:10.4314/ovj.v7i2.17



Vancouver/ICMJE Style

Syamalima Dube, Henry Chionuma, Amr Matoq, Ruham Alshiekh-Nasany, Lynn Abbott, Bernard J. Poiesz, Dipak K. Dube. Expression of various sarcomeric tropomyosin isoforms in equine striated muscles. Open Vet J. (2017), [cited February 28, 2024]; 7(2): 180-191. doi:10.4314/ovj.v7i2.17



Harvard Style

Syamalima Dube, Henry Chionuma, Amr Matoq, Ruham Alshiekh-Nasany, Lynn Abbott, Bernard J. Poiesz, Dipak K. Dube (2017) Expression of various sarcomeric tropomyosin isoforms in equine striated muscles. Open Vet J, 7 (2), 180-191. doi:10.4314/ovj.v7i2.17



Turabian Style

Syamalima Dube, Henry Chionuma, Amr Matoq, Ruham Alshiekh-Nasany, Lynn Abbott, Bernard J. Poiesz, Dipak K. Dube. 2017. Expression of various sarcomeric tropomyosin isoforms in equine striated muscles. Open Veterinary Journal, 7 (2), 180-191. doi:10.4314/ovj.v7i2.17



Chicago Style

Syamalima Dube, Henry Chionuma, Amr Matoq, Ruham Alshiekh-Nasany, Lynn Abbott, Bernard J. Poiesz, Dipak K. Dube. "Expression of various sarcomeric tropomyosin isoforms in equine striated muscles." Open Veterinary Journal 7 (2017), 180-191. doi:10.4314/ovj.v7i2.17



MLA (The Modern Language Association) Style

Syamalima Dube, Henry Chionuma, Amr Matoq, Ruham Alshiekh-Nasany, Lynn Abbott, Bernard J. Poiesz, Dipak K. Dube. "Expression of various sarcomeric tropomyosin isoforms in equine striated muscles." Open Veterinary Journal 7.2 (2017), 180-191. Print. doi:10.4314/ovj.v7i2.17



APA (American Psychological Association) Style

Syamalima Dube, Henry Chionuma, Amr Matoq, Ruham Alshiekh-Nasany, Lynn Abbott, Bernard J. Poiesz, Dipak K. Dube (2017) Expression of various sarcomeric tropomyosin isoforms in equine striated muscles. Open Veterinary Journal, 7 (2), 180-191. doi:10.4314/ovj.v7i2.17