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2025, Vol: 15, Issue: 3
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DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period

M. Tharwat1, 2, D. Endoh3 And S. Oikawa1, *.


Cited By:12

Abstract
This study was designed to investigate the apoptotic process in peripheral blood mononuclear cells (PBMC) and polymorphonuclear neutrophil leukocytes (PMN) in dairy cattle during the transition period. Blood samples were collected from 4 dairy cattle at 3 weeks before the expected parturition (wk -3), parturition (wk 0) and 3 weeks after parturition (wk +3). The DNA damage of PBMC and PMN was evaluated based on the comet assay using visual scoring (arbitrary units). Undamaged DNA remained within the core (score 0) and the broken DNA migrated from the core towards the anode forming the tail of a comet (scores 1-4). Significantly higher scores in PBMC at wk 0 and wk +3 were observed compared with those in PMN although there were no significant changes of scores in either cell type during the experimental period. It is suggested that the apoptotic rate of PBMC is accelerated compared with that of PMC during the transition period.

Key words: Apoptosis, Comet assay, Cow, Leukocytes, Transition period.


 
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How to Cite this Article
Pubmed Style

Tharwat M, , Oikawa DEAS. DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period. Open Vet J. 2012; 2(0): 65-68. doi:10.5455/OVJ.2012.v2.i0.p65

Web Style

Tharwat M, , Oikawa DEAS. DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period. https://www.openveterinaryjournal.com/?mno=302656789 [Access: April 28, 2025]. doi:10.5455/OVJ.2012.v2.i0.p65

AMA (American Medical Association) Style

Tharwat M, , Oikawa DEAS. DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period. Open Vet J. 2012; 2(0): 65-68. doi:10.5455/OVJ.2012.v2.i0.p65


Vancouver/ICMJE Style

Tharwat M, , Oikawa DEAS. DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period. Open Vet J. (2012), [cited April 28, 2025]; 2(0): 65-68. doi:10.5455/OVJ.2012.v2.i0.p65


Harvard Style

Tharwat, M., , . & Oikawa, . D. E. A. S. (2012) DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period. Open Vet J, 2 (0), 65-68. doi:10.5455/OVJ.2012.v2.i0.p65


Turabian Style

Tharwat, M., , and D. Endoh And S. Oikawa. 2012. DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period. Open Veterinary Journal, 2 (0), 65-68. doi:10.5455/OVJ.2012.v2.i0.p65


Chicago Style

Tharwat, M., , and D. Endoh And S. Oikawa. "DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period." Open Veterinary Journal 2 (2012), 65-68. doi:10.5455/OVJ.2012.v2.i0.p65


MLA (The Modern Language Association) Style

Tharwat, M., , and D. Endoh And S. Oikawa. "DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period." Open Veterinary Journal 2.0 (2012), 65-68. Print. doi:10.5455/OVJ.2012.v2.i0.p65


APA (American Psychological Association) Style

Tharwat, M., , . & Oikawa, . D. E. A. S. (2012) DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period. Open Veterinary Journal, 2 (0), 65-68. doi:10.5455/OVJ.2012.v2.i0.p65


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