E-ISSN 2218-6050 | ISSN 2226-4485
 

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DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period

M. Tharwat1,2, D. Endoh3 and S. Oikawa1,*.


Abstract
This study was designed to investigate the apoptotic process in peripheral blood mononuclear cells (PBMC) and polymorphonuclear neutrophil leukocytes (PMN) in dairy cattle during the transition period. Blood samples were collected from 4 dairy cattle at 3 weeks before the expected parturition (wk -3), parturition (wk 0) and 3 weeks after parturition (wk +3). The DNA damage of PBMC and PMN was evaluated based on the comet assay using visual scoring (arbitrary units). Undamaged DNA remained within the core (score 0) and the broken DNA migrated from the core towards the anode forming the tail of a comet (scores 1-4). Significantly higher scores in PBMC at wk 0 and wk +3 were observed compared with those in PMN although there were no significant changes of scores in either cell type during the experimental period. It is suggested that the apoptotic rate of PBMC is accelerated compared with that of PMC during the transition period.

Key words: Apoptosis, Comet assay, Cow, Leukocytes, Transition period.


 
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Pubmed Style

M. Tharwat,, D. Endoh and S. Oikawa,. DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period. Open Vet J. 2012; 2(0): 65-68. doi:10.5455/OVJ.2012.v2.i0.p65


Web Style

M. Tharwat,, D. Endoh and S. Oikawa,. DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period. https://www.openveterinaryjournal.com/?mno=302656789 [Access: February 28, 2024]. doi:10.5455/OVJ.2012.v2.i0.p65


AMA (American Medical Association) Style

M. Tharwat,, D. Endoh and S. Oikawa,. DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period. Open Vet J. 2012; 2(0): 65-68. doi:10.5455/OVJ.2012.v2.i0.p65



Vancouver/ICMJE Style

M. Tharwat,, D. Endoh and S. Oikawa,. DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period. Open Vet J. (2012), [cited February 28, 2024]; 2(0): 65-68. doi:10.5455/OVJ.2012.v2.i0.p65



Harvard Style

M. Tharwat,, D. Endoh and S. Oikawa, (2012) DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period. Open Vet J, 2 (0), 65-68. doi:10.5455/OVJ.2012.v2.i0.p65



Turabian Style

M. Tharwat,, D. Endoh and S. Oikawa,. 2012. DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period. Open Veterinary Journal, 2 (0), 65-68. doi:10.5455/OVJ.2012.v2.i0.p65



Chicago Style

M. Tharwat,, D. Endoh and S. Oikawa,. "DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period." Open Veterinary Journal 2 (2012), 65-68. doi:10.5455/OVJ.2012.v2.i0.p65



MLA (The Modern Language Association) Style

M. Tharwat,, D. Endoh and S. Oikawa,. "DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period." Open Veterinary Journal 2.0 (2012), 65-68. Print. doi:10.5455/OVJ.2012.v2.i0.p65



APA (American Psychological Association) Style

M. Tharwat,, D. Endoh and S. Oikawa, (2012) DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period. Open Veterinary Journal, 2 (0), 65-68. doi:10.5455/OVJ.2012.v2.i0.p65