E-ISSN 2218-6050 | ISSN 2226-4485
 

Original Article


More Cell Culture Passaged Camelpox virus Sequences Found Resembling those of Vaccinia Virus

Abdelmalik Ibrahim Khalafalla, Salama S Al Muhairi, Hassan Zackaria Ali Ishag, Mohamed A Al Hosani.


Cited By:5

Abstract
Background: Camelpox is the most infectious and economically important disease of camelids that causes significant morbidity and mortality rates. Several live attenuated vaccines against camelpox virus (CMLV) are produced worldwide by passaging field isolates in cell culture. Sequence of a high passage Saudi isolate of CMLV was previously found closely resembled Vaccinia virus (VACV).
Aim: To determine whether other high cell culture passage CMLV isolates are genetically resemble VACV and further to explore the possible mechanism of the resemblance.
Methods: We performed PCR and DNA sequence analysis of ATIP, L1R and ORF 185 genes on different cell culture passage levels of a field isolate, two high passage vaccines, wild-type and reference strains of CMLV.
Results: We demonstrate that additional two high passage attenuated vaccine candidate from Sudan and UAE likewise contain sequences resembling VACV more than CMLV. Furthermore, sequence analysis of the A-type inclusion body protein (ATIP) gene of selected virus passages in cell culture revealed that the shift to VACV-like occurred between passage 11 and 20 and up to the 10th passage the genome still resembles wild-type virus. This observation was further confirmed by recombination analysis which indicated recombination events at ATIP and ORF185 genes occurred at higher passages.
Conclusion: We confirmed that cell culture passage CMLV turn to resemble VACV after cell culture passage and concluded that the resemblance may not be a result of contamination or misidentification as previously thought but could be due to recombination events that occurred during the passage process.

Key words: Camelpox virus; Cell culture passage; Phylogenetic analysis


 
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How to Cite this Article
Pubmed Style

Khalafalla AI, Muhairi SSA, Ishag HZA, Hosani MAA. More Cell Culture Passaged Camelpox virus Sequences Found Resembling those of Vaccinia Virus. Open Vet J. 2020; 10(2): 144-156. doi:10.4314/ovj.v10i2.4


Web Style

Khalafalla AI, Muhairi SSA, Ishag HZA, Hosani MAA. More Cell Culture Passaged Camelpox virus Sequences Found Resembling those of Vaccinia Virus. https://www.openveterinaryjournal.com/?mno=66095 [Access: February 15, 2024]. doi:10.4314/ovj.v10i2.4


AMA (American Medical Association) Style

Khalafalla AI, Muhairi SSA, Ishag HZA, Hosani MAA. More Cell Culture Passaged Camelpox virus Sequences Found Resembling those of Vaccinia Virus. Open Vet J. 2020; 10(2): 144-156. doi:10.4314/ovj.v10i2.4



Vancouver/ICMJE Style

Khalafalla AI, Muhairi SSA, Ishag HZA, Hosani MAA. More Cell Culture Passaged Camelpox virus Sequences Found Resembling those of Vaccinia Virus. Open Vet J. (2020), [cited February 15, 2024]; 10(2): 144-156. doi:10.4314/ovj.v10i2.4



Harvard Style

Khalafalla, A. I., Muhairi, . S. S. A., Ishag, . H. Z. A. & Hosani, . M. A. A. (2020) More Cell Culture Passaged Camelpox virus Sequences Found Resembling those of Vaccinia Virus. Open Vet J, 10 (2), 144-156. doi:10.4314/ovj.v10i2.4



Turabian Style

Khalafalla, Abdelmalik Ibrahim, Salama S Al Muhairi, Hassan Zackaria Ali Ishag, and Mohamed A Al Hosani. 2020. More Cell Culture Passaged Camelpox virus Sequences Found Resembling those of Vaccinia Virus. Open Veterinary Journal, 10 (2), 144-156. doi:10.4314/ovj.v10i2.4



Chicago Style

Khalafalla, Abdelmalik Ibrahim, Salama S Al Muhairi, Hassan Zackaria Ali Ishag, and Mohamed A Al Hosani. "More Cell Culture Passaged Camelpox virus Sequences Found Resembling those of Vaccinia Virus." Open Veterinary Journal 10 (2020), 144-156. doi:10.4314/ovj.v10i2.4



MLA (The Modern Language Association) Style

Khalafalla, Abdelmalik Ibrahim, Salama S Al Muhairi, Hassan Zackaria Ali Ishag, and Mohamed A Al Hosani. "More Cell Culture Passaged Camelpox virus Sequences Found Resembling those of Vaccinia Virus." Open Veterinary Journal 10.2 (2020), 144-156. Print. doi:10.4314/ovj.v10i2.4



APA (American Psychological Association) Style

Khalafalla, A. I., Muhairi, . S. S. A., Ishag, . H. Z. A. & Hosani, . M. A. A. (2020) More Cell Culture Passaged Camelpox virus Sequences Found Resembling those of Vaccinia Virus. Open Veterinary Journal, 10 (2), 144-156. doi:10.4314/ovj.v10i2.4