E-ISSN 2218-6050 | ISSN 2226-4485
 

Original Article


Efficacy of two primer sets used in Sex Identification of Rufous-winged Buzzard (Butastur liventer).

Paitoon Kaewhom, Kanokrat Srikijkasemwat.


Abstract
Background: The rufous-winged buzzard (Butastur liventer) belongs to the Accipitriformes, which are monomorphic, resulting in difficulty to identify the gender. However, sex determination is important for predicting the sex ratio this buzzard in nature in order to avoid its extinction.
Aim: We aimed to develop a primer set, able to sex the rufous-winged buzzard through PCR amplification, and compare the efficacy of two sets of primers by using PCR technique.
Methods: In the following, sensitivity refers to the smallest DNA concentration that allowed us to accurately sex a bird and specificity refers to the ability to clearly distinguish the sex based on visual appearance of the bands.
Blood samples were collected from captive buzzards. The DNA was extracted from them and was diluted to 50, 25, 10, 5, 2.5, 1.67 and 1 ng/µL. Two sets of primers, including P2/NP/MP and 2550F/2718R, were used to amplify the CHD gene of known gender buzzards using the PCR process to determine gender and to compare their sensitivity. To measure specificity, both primers were used to amplify CHD gene fragment of other unknown gender birds.
Results: The lowest concentration of the DNA template that P2/NP/MP could amplify DNA fragments was 1 ng/µL, and this set of primers could identify the gender of all birds correctly, giving 100% specificity. On the other hand, the 2550F/2718R could amplify the DNA fragments from 5 ng/µL, and it had only 78% specificity.
Conclusion: The P2/NP/MP primer set was able to correctly identify the gender of rufous-winged buzzard through PCR amplification with high specificity and sensitivity.

Key words: CHD gene, PCR technique, Gender identification, Birds


 
ARTICLE TOOLS
Abstract
PDF Fulltext
HTML Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Paitoon Kaewhom
Articles by Kanokrat Srikijkasemwat
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Kaewhom P, Srikijkasemwat K, . Efficacy of two primer sets used in Sex Identification of Rufous-winged Buzzard (Butastur liventer).. Open Vet J. 2021; 11(4): 581-586. doi:10.5455/OVJ.2021.v11.i4.7


Web Style

Kaewhom P, Srikijkasemwat K, . Efficacy of two primer sets used in Sex Identification of Rufous-winged Buzzard (Butastur liventer).. https://www.openveterinaryjournal.com/?mno=91008 [Access: February 14, 2024]. doi:10.5455/OVJ.2021.v11.i4.7


AMA (American Medical Association) Style

Kaewhom P, Srikijkasemwat K, . Efficacy of two primer sets used in Sex Identification of Rufous-winged Buzzard (Butastur liventer).. Open Vet J. 2021; 11(4): 581-586. doi:10.5455/OVJ.2021.v11.i4.7



Vancouver/ICMJE Style

Kaewhom P, Srikijkasemwat K, . Efficacy of two primer sets used in Sex Identification of Rufous-winged Buzzard (Butastur liventer).. Open Vet J. (2021), [cited February 14, 2024]; 11(4): 581-586. doi:10.5455/OVJ.2021.v11.i4.7



Harvard Style

Kaewhom, P., Srikijkasemwat, K. & (2021) Efficacy of two primer sets used in Sex Identification of Rufous-winged Buzzard (Butastur liventer).. Open Vet J, 11 (4), 581-586. doi:10.5455/OVJ.2021.v11.i4.7



Turabian Style

Kaewhom, Paitoon, Kanokrat Srikijkasemwat, and . 2021. Efficacy of two primer sets used in Sex Identification of Rufous-winged Buzzard (Butastur liventer).. Open Veterinary Journal, 11 (4), 581-586. doi:10.5455/OVJ.2021.v11.i4.7



Chicago Style

Kaewhom, Paitoon, Kanokrat Srikijkasemwat, and . "Efficacy of two primer sets used in Sex Identification of Rufous-winged Buzzard (Butastur liventer).." Open Veterinary Journal 11 (2021), 581-586. doi:10.5455/OVJ.2021.v11.i4.7



MLA (The Modern Language Association) Style

Kaewhom, Paitoon, Kanokrat Srikijkasemwat, and . "Efficacy of two primer sets used in Sex Identification of Rufous-winged Buzzard (Butastur liventer).." Open Veterinary Journal 11.4 (2021), 581-586. Print. doi:10.5455/OVJ.2021.v11.i4.7



APA (American Psychological Association) Style

Kaewhom, P., Srikijkasemwat, K. & (2021) Efficacy of two primer sets used in Sex Identification of Rufous-winged Buzzard (Butastur liventer).. Open Veterinary Journal, 11 (4), 581-586. doi:10.5455/OVJ.2021.v11.i4.7