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Research Article


Molecular detection of extended-spectrum β-lactamase-producing Escherichia coli from bat caves on Lombok Island

Yolla Rona Mustika, Kurnia Nisa Kinasih, Mustofa Helmi Effendi, Yulianna Puspitasari, Shendy Canadya Kurniawan, Aswin Rafif Khairullah, Muhammad Esa Erlang Samodra, Abdullah Hasib, Alfiana Laili Dwi Agustin, Ikechukwu Benjamin Moses, Otto Sahat Martua Silaen.


Abstract
Background:
The discovery of antibiotic-resistant Enterobacteriaceae bacteria in wild animals is an indication of their potential for wildlife as a reservoir. Bats are natural reservoir hosts and a source of infection for several microorganisms and have the potential to become vectors for the spread of zoonotic diseases.
Aim:
A study was conducted based on these characteristics to identify and detect the blaTEM gene in Eschericia coli isolated from bat excrements in Tanjung Ringgit Cave, East Lombok.
Methods:
Bat faecal samples were firstly inoculated onto EMB agar media. Recovered bacterial isolates were further characterized using standard microbiological techniques. Antimicrobial susceptibility testing was done using the Kirby-Bauer disc diffusion method. blaTEM gene detection was carried out using polymerase chain reaction (PCR).
Results:
Out of the 150 bat fecal samples obtained from Tanjung Ringgit cave, Lombok Island, Indonesia, 56 (37 %) were positive for E. coli. Eight (8) out of the 56 E. coli isolates that underwent antimicrobial susceptibility testing using the disc diffusion method were confirmed to be multidrug-resistant as they exhibited resistance to at least three different classes of antibiotics. Out of the eight (8) MDR E. coli isolates recovered from fecal samples of bats, 2 (two) harbored the blaTEM gene.
Conclusion:
The discovery of the blaTEM gene in bat fecal samples indicates the potential for wild animals, especially bats, to spread ESBL resistance genes to the environment and to humans.

Key words: Escherichia coli, Multidrug resistance, Bats, blaTEM, Human health


 
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How to Cite this Article
Pubmed Style

Mustika YR, Kinasih KN, Effendi MH, Puspitasari Y, Kurniawan SC, Khairullah AR, Samodra MEE, Hasib A, Agustin ALD, Moses IB, Silaen OSM. Molecular detection of extended-spectrum β-lactamase-producing Escherichia coli from bat caves on Lombok Island. Open Vet J. 2024; 14(2): 699-706. doi:10.5455/OVJ.2024.v14.i2.10

Web Style

Mustika YR, Kinasih KN, Effendi MH, Puspitasari Y, Kurniawan SC, Khairullah AR, Samodra MEE, Hasib A, Agustin ALD, Moses IB, Silaen OSM. Molecular detection of extended-spectrum β-lactamase-producing Escherichia coli from bat caves on Lombok Island. https://www.openveterinaryjournal.com/?mno=178548 [Access: April 21, 2024]. doi:10.5455/OVJ.2024.v14.i2.10

AMA (American Medical Association) Style

Mustika YR, Kinasih KN, Effendi MH, Puspitasari Y, Kurniawan SC, Khairullah AR, Samodra MEE, Hasib A, Agustin ALD, Moses IB, Silaen OSM. Molecular detection of extended-spectrum β-lactamase-producing Escherichia coli from bat caves on Lombok Island. Open Vet J. 2024; 14(2): 699-706. doi:10.5455/OVJ.2024.v14.i2.10


Vancouver/ICMJE Style

Mustika YR, Kinasih KN, Effendi MH, Puspitasari Y, Kurniawan SC, Khairullah AR, Samodra MEE, Hasib A, Agustin ALD, Moses IB, Silaen OSM. Molecular detection of extended-spectrum β-lactamase-producing Escherichia coli from bat caves on Lombok Island. Open Vet J. (2024), [cited April 21, 2024]; 14(2): 699-706. doi:10.5455/OVJ.2024.v14.i2.10


Harvard Style

Mustika, Y. R., Kinasih, . K. N., Effendi, . M. H., Puspitasari, . Y., Kurniawan, . S. C., Khairullah, . A. R., Samodra, . M. E. E., Hasib, . A., Agustin, . A. L. D., Moses, . I. B. & Silaen, . O. S. M. (2024) Molecular detection of extended-spectrum β-lactamase-producing Escherichia coli from bat caves on Lombok Island. Open Vet J, 14 (2), 699-706. doi:10.5455/OVJ.2024.v14.i2.10


Turabian Style

Mustika, Yolla Rona, Kurnia Nisa Kinasih, Mustofa Helmi Effendi, Yulianna Puspitasari, Shendy Canadya Kurniawan, Aswin Rafif Khairullah, Muhammad Esa Erlang Samodra, Abdullah Hasib, Alfiana Laili Dwi Agustin, Ikechukwu Benjamin Moses, and Otto Sahat Martua Silaen. 2024. Molecular detection of extended-spectrum β-lactamase-producing Escherichia coli from bat caves on Lombok Island. Open Veterinary Journal, 14 (2), 699-706. doi:10.5455/OVJ.2024.v14.i2.10


Chicago Style

Mustika, Yolla Rona, Kurnia Nisa Kinasih, Mustofa Helmi Effendi, Yulianna Puspitasari, Shendy Canadya Kurniawan, Aswin Rafif Khairullah, Muhammad Esa Erlang Samodra, Abdullah Hasib, Alfiana Laili Dwi Agustin, Ikechukwu Benjamin Moses, and Otto Sahat Martua Silaen. "Molecular detection of extended-spectrum β-lactamase-producing Escherichia coli from bat caves on Lombok Island." Open Veterinary Journal 14 (2024), 699-706. doi:10.5455/OVJ.2024.v14.i2.10


MLA (The Modern Language Association) Style

Mustika, Yolla Rona, Kurnia Nisa Kinasih, Mustofa Helmi Effendi, Yulianna Puspitasari, Shendy Canadya Kurniawan, Aswin Rafif Khairullah, Muhammad Esa Erlang Samodra, Abdullah Hasib, Alfiana Laili Dwi Agustin, Ikechukwu Benjamin Moses, and Otto Sahat Martua Silaen. "Molecular detection of extended-spectrum β-lactamase-producing Escherichia coli from bat caves on Lombok Island." Open Veterinary Journal 14.2 (2024), 699-706. Print. doi:10.5455/OVJ.2024.v14.i2.10


APA (American Psychological Association) Style

Mustika, Y. R., Kinasih, . K. N., Effendi, . M. H., Puspitasari, . Y., Kurniawan, . S. C., Khairullah, . A. R., Samodra, . M. E. E., Hasib, . A., Agustin, . A. L. D., Moses, . I. B. & Silaen, . O. S. M. (2024) Molecular detection of extended-spectrum β-lactamase-producing Escherichia coli from bat caves on Lombok Island. Open Veterinary Journal, 14 (2), 699-706. doi:10.5455/OVJ.2024.v14.i2.10


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