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Research Article


Effects of fermented Crescentia cujete L. on the profile of haematology, clinical chemistry and circulatory CD4+/CD8+ in Sprague Dawley rats

Agustina Dwi Wijayanti, Yos Adi Prakoso, Krestel Joy Viernes Isla.


Abstract
Background:
The calabash (Crescentia cujete L.) is a tropical fruit that offers numerous health benefits. Its fermented form (FC) has been found to affect the neurological system positively. However, its impact on haematology, clinical chemistry, and CD4+ and CD8+ levels has yet to be documented.
Aim:
Therefore, this study aims to examine the effects of FC on haematology, clinical chemistry, and the levels of CD4+ and CD8+ in the circulatory system using rat models.
Methods:
This study used twenty male Sprague Dawley rats. The rats were divided into group 1 (0 mg/kg BW FC), group 2 (50 mg/kg BW FC), group 3 (500 mg/kg BW FC), and group 4 (2,000 mg/kg BW FC). The treatment was administered using a gastric probe once daily for 14 days. On day 15, the blood samples were collected and tested against haematology, clinical chemistry, quality of the erythrocytes, and CD4+/CD8+. The data was then analysed using SPSS with p-value at 0.05.
Results:
The conducted study demonstrated that the utilisation of FC at varying doses did not have a significant impact on the haematological profile changes (p≥0.05), except for total lymphocytes and a decrease in the neutrophils/lymphocytes (N/L) ratio (p≤0.05). Furthermore, FC did not influence the changes in clinical chemistry, circulatory protein, and electrolyte levels in rat models compared to the control (p≥0.05). The utilisation of FC decreased the percentage of haemolysis and elevated the ATP concentration (p≤0.05). Additionally, the use of FC led to a significant increase in CD4+ and the ratio of CD4+/CD8+ (p≤0.05), while no significant effect was observed regarding CD8+ (p≥0.05).
Conclusion:
The study highlighted FC’s beneficial effects on the haemorheology and immune system, specifically on the decrease in the percentage of haemolysis, elevated ATP concentration, number of lymphocytes, ratio N/L, CD4+, and the CD4+/CD8+ ratio, without causing significant changes to the haematological and clinical chemistry profiles in rat models.

Key words: CD4+, CD8+, Clinical chemistry, Fermented Crescentia cujete (L), Haematology


 
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How to Cite this Article
Pubmed Style

Wijayanti AD, Prakoso YA, Isla KJV. Effects of fermented Crescentia cujete L. on the profile of haematology, clinical chemistry and circulatory CD4+/CD8+ in Sprague Dawley rats. Open Vet J. 2024; 14(9): 2475-2483. doi:10.5455/OVJ.2024.v14.i9.36


Web Style

Wijayanti AD, Prakoso YA, Isla KJV. Effects of fermented Crescentia cujete L. on the profile of haematology, clinical chemistry and circulatory CD4+/CD8+ in Sprague Dawley rats. https://www.openveterinaryjournal.com/?mno=207513 [Access: November 07, 2024]. doi:10.5455/OVJ.2024.v14.i9.36


AMA (American Medical Association) Style

Wijayanti AD, Prakoso YA, Isla KJV. Effects of fermented Crescentia cujete L. on the profile of haematology, clinical chemistry and circulatory CD4+/CD8+ in Sprague Dawley rats. Open Vet J. 2024; 14(9): 2475-2483. doi:10.5455/OVJ.2024.v14.i9.36



Vancouver/ICMJE Style

Wijayanti AD, Prakoso YA, Isla KJV. Effects of fermented Crescentia cujete L. on the profile of haematology, clinical chemistry and circulatory CD4+/CD8+ in Sprague Dawley rats. Open Vet J. (2024), [cited November 07, 2024]; 14(9): 2475-2483. doi:10.5455/OVJ.2024.v14.i9.36



Harvard Style

Wijayanti, A. D., Prakoso, . Y. A. & Isla, . K. J. V. (2024) Effects of fermented Crescentia cujete L. on the profile of haematology, clinical chemistry and circulatory CD4+/CD8+ in Sprague Dawley rats. Open Vet J, 14 (9), 2475-2483. doi:10.5455/OVJ.2024.v14.i9.36



Turabian Style

Wijayanti, Agustina Dwi, Yos Adi Prakoso, and Krestel Joy Viernes Isla. 2024. Effects of fermented Crescentia cujete L. on the profile of haematology, clinical chemistry and circulatory CD4+/CD8+ in Sprague Dawley rats. Open Veterinary Journal, 14 (9), 2475-2483. doi:10.5455/OVJ.2024.v14.i9.36



Chicago Style

Wijayanti, Agustina Dwi, Yos Adi Prakoso, and Krestel Joy Viernes Isla. "Effects of fermented Crescentia cujete L. on the profile of haematology, clinical chemistry and circulatory CD4+/CD8+ in Sprague Dawley rats." Open Veterinary Journal 14 (2024), 2475-2483. doi:10.5455/OVJ.2024.v14.i9.36



MLA (The Modern Language Association) Style

Wijayanti, Agustina Dwi, Yos Adi Prakoso, and Krestel Joy Viernes Isla. "Effects of fermented Crescentia cujete L. on the profile of haematology, clinical chemistry and circulatory CD4+/CD8+ in Sprague Dawley rats." Open Veterinary Journal 14.9 (2024), 2475-2483. Print. doi:10.5455/OVJ.2024.v14.i9.36



APA (American Psychological Association) Style

Wijayanti, A. D., Prakoso, . Y. A. & Isla, . K. J. V. (2024) Effects of fermented Crescentia cujete L. on the profile of haematology, clinical chemistry and circulatory CD4+/CD8+ in Sprague Dawley rats. Open Veterinary Journal, 14 (9), 2475-2483. doi:10.5455/OVJ.2024.v14.i9.36