E-ISSN 2218-6050 | ISSN 2226-4485
 
2024, Vol: 14, Issue: 11
14 / 11Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submission Form

Short Communication


Study of horn flies as vectors of bovine leukemia virus

Carlos Javier Panei, Alejandra Edith Larsen, Nadia Analia Fuentealba, German Ernesto Metz, Maria Gabriela Echeverria, Cecilia Monica Galosi, Alejandro Rafael Valera.


Cited By:27

Abstract
Bovine leukemia virus (BLV) is the agent responsible for enzootic bovine leukosis, the most common neoplastic disease in cattle. The horn fly, a major hematophagous pest of cattle, is able to transmit different diseases in cattle. However, its implication in BLV transmission under a natural environment is still discussed. The objectives of this work were to determine the presence of BLV in horn flies (by sequencing) and to evaluate the ability of horn flies to transmit BLV to cattle (through an experimental assay under a natural environment). To demonstrate the presence of BLV in the flies, 40 horn flies were collected from a BLV-positive cow with a sweep net and ten pools with four horn-fly mouthparts each were prepared. The presence of BLV was determined by nested PCR and sequencing. To demonstrate BLV transmission, other 40 flies were collected from the same BLV-positive cow with a sweep net. Eight homogenates containing five horn-fly mouthparts each were prepared and injected to eight cows of different breeds, and blood samples were collected every 21 days. Then, to evaluate the ability of horn flies to transmit BLV to grazing cattle under natural conditions, both infected and uninfected cattle from the experimental transmission assay were kept together in the same paddock with more than 200 horn flies per animal for 120 days. Blood samples were collected every 20 days and the number of flies determined. The sequencing results confirmed the presence of the provirus in horn flies. The results also confirmed that BLV transmission is a possible event, at least experimentally. However, the role of horn flies as vectors of BLV under a natural grazing system is still discussed.

Key words: Haematobia irritans, blood-sucking insect, BLV transmission, enzootic bovine leucosis, horn flies


 
ARTICLE TOOLS
Abstract
PDF Fulltext
HTML Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Carlos Javier Panei
Articles by Alejandra Edith Larsen
Articles by Nadia Analia Fuentealba
Articles by German Ernesto Metz
Articles by Maria Gabriela Echeverria
Articles by Cecilia Monica Galosi
Articles by Alejandro Rafael Valera
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Carlos Javier Panei, Alejandra Edith Larsen, Nadia Analia Fuentealba, German Ernesto Metz, Maria Gabriela Echeverria, Cecilia Monica Galosi, Alejandro Rafael Valera. Study of horn flies as vectors of bovine leukemia virus. Open Vet J. 2019; 9(1): 33-37. doi:10.4314/ovj.v9i1.6

Web Style

Carlos Javier Panei, Alejandra Edith Larsen, Nadia Analia Fuentealba, German Ernesto Metz, Maria Gabriela Echeverria, Cecilia Monica Galosi, Alejandro Rafael Valera. Study of horn flies as vectors of bovine leukemia virus. https://www.openveterinaryjournal.com/?mno=302643015 [Access: December 04, 2024]. doi:10.4314/ovj.v9i1.6

AMA (American Medical Association) Style

Carlos Javier Panei, Alejandra Edith Larsen, Nadia Analia Fuentealba, German Ernesto Metz, Maria Gabriela Echeverria, Cecilia Monica Galosi, Alejandro Rafael Valera. Study of horn flies as vectors of bovine leukemia virus. Open Vet J. 2019; 9(1): 33-37. doi:10.4314/ovj.v9i1.6


Vancouver/ICMJE Style

Carlos Javier Panei, Alejandra Edith Larsen, Nadia Analia Fuentealba, German Ernesto Metz, Maria Gabriela Echeverria, Cecilia Monica Galosi, Alejandro Rafael Valera. Study of horn flies as vectors of bovine leukemia virus. Open Vet J. (2019), [cited December 04, 2024]; 9(1): 33-37. doi:10.4314/ovj.v9i1.6


Harvard Style

Carlos Javier Panei, Alejandra Edith Larsen, Nadia Analia Fuentealba, German Ernesto Metz, Maria Gabriela Echeverria, Cecilia Monica Galosi, Alejandro Rafael Valera (2019) Study of horn flies as vectors of bovine leukemia virus. Open Vet J, 9 (1), 33-37. doi:10.4314/ovj.v9i1.6


Turabian Style

Carlos Javier Panei, Alejandra Edith Larsen, Nadia Analia Fuentealba, German Ernesto Metz, Maria Gabriela Echeverria, Cecilia Monica Galosi, Alejandro Rafael Valera. 2019. Study of horn flies as vectors of bovine leukemia virus. Open Veterinary Journal, 9 (1), 33-37. doi:10.4314/ovj.v9i1.6


Chicago Style

Carlos Javier Panei, Alejandra Edith Larsen, Nadia Analia Fuentealba, German Ernesto Metz, Maria Gabriela Echeverria, Cecilia Monica Galosi, Alejandro Rafael Valera. "Study of horn flies as vectors of bovine leukemia virus." Open Veterinary Journal 9 (2019), 33-37. doi:10.4314/ovj.v9i1.6


MLA (The Modern Language Association) Style

Carlos Javier Panei, Alejandra Edith Larsen, Nadia Analia Fuentealba, German Ernesto Metz, Maria Gabriela Echeverria, Cecilia Monica Galosi, Alejandro Rafael Valera. "Study of horn flies as vectors of bovine leukemia virus." Open Veterinary Journal 9.1 (2019), 33-37. Print. doi:10.4314/ovj.v9i1.6


APA (American Psychological Association) Style

Carlos Javier Panei, Alejandra Edith Larsen, Nadia Analia Fuentealba, German Ernesto Metz, Maria Gabriela Echeverria, Cecilia Monica Galosi, Alejandro Rafael Valera (2019) Study of horn flies as vectors of bovine leukemia virus. Open Veterinary Journal, 9 (1), 33-37. doi:10.4314/ovj.v9i1.6


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldagpublisher.com 

Email: ibrahim.eldaghayes@vetmed.edu.ly