E-ISSN 2218-6050 | ISSN 2226-4485
 

Original Article


Serum proteomic analysis reveals the differential dose effects of crocodile oil from Crocodylus siamensis on energy metabolism in rats

Wirasak Fungfuang.

Abstract
Background: Dietary fat composition is a potential major factor affecting energy metabolism. Crocodile oil (CO) is rich in mono- and poly-unsaturated fatty acids exhibiting anti-inflammatory and healing properties.
Aim: This study investigated different levels of CO consumption on alterations and expression of proteins involved in energy metabolism in rats.
Methods: Twenty-one male Sprague-Dawley rats were divided into three groups and administered sterile water (N) or different doses of CO (1% or 3% [v/w] CO) orally once daily for 8 weeks. Body weight gain, food intake, energy intake, blood lipid profiles, and serum energy-related metabolites were determined. The serum proteome was analyzed using shotgun proteomics, and the functions of several candidate proteins were classified using PANTHER software.
Results: There were no significant differences in body weight or energy intake were observed between groups. However, both CO-treated groups showed significantly decreased serum triglyceride (TG) levels (p<0.05). Moreover, post-treatment serum TG levels in the 1% CO group were significantly lower than pre-treatment compared with other groups. The serum oxaloacetate level was also significantly higher in both CO groups than in the N group. The proteomic analysis classified 4,525 serum proteins and revealed more unique proteins involved in cellular metabolic activity in both CO-treated groups than in the N group. Self-organizing tree algorithm clustering of 295 shared differentially expressed proteins in both CO-treatment groups showed that upregulated hyper-expressed protein clusters in both CO groups were associated with catalytic activity and molecular activity on the same levels.
Conclusion: CO simultaneously enhances energy metabolism and improves lipid profiles.

Key words: crocodile oil; energy metabolism; serum; proteomics; rat


 
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Pubmed Style

Fungfuang W, . Serum proteomic analysis reveals the differential dose effects of crocodile oil from Crocodylus siamensis on energy metabolism in rats. Open Vet J. 2022; 12(5): 697-708. doi:10.5455/OVJ.2022.v12.i5.15


Web Style

Fungfuang W, . Serum proteomic analysis reveals the differential dose effects of crocodile oil from Crocodylus siamensis on energy metabolism in rats. https://www.openveterinaryjournal.com/?mno=45178 [Access: November 28, 2022]. doi:10.5455/OVJ.2022.v12.i5.15


AMA (American Medical Association) Style

Fungfuang W, . Serum proteomic analysis reveals the differential dose effects of crocodile oil from Crocodylus siamensis on energy metabolism in rats. Open Vet J. 2022; 12(5): 697-708. doi:10.5455/OVJ.2022.v12.i5.15



Vancouver/ICMJE Style

Fungfuang W, . Serum proteomic analysis reveals the differential dose effects of crocodile oil from Crocodylus siamensis on energy metabolism in rats. Open Vet J. (2022), [cited November 28, 2022]; 12(5): 697-708. doi:10.5455/OVJ.2022.v12.i5.15



Harvard Style

Fungfuang, W. & (2022) Serum proteomic analysis reveals the differential dose effects of crocodile oil from Crocodylus siamensis on energy metabolism in rats. Open Vet J, 12 (5), 697-708. doi:10.5455/OVJ.2022.v12.i5.15



Turabian Style

Fungfuang, Wirasak, and . 2022. Serum proteomic analysis reveals the differential dose effects of crocodile oil from Crocodylus siamensis on energy metabolism in rats. Open Veterinary Journal, 12 (5), 697-708. doi:10.5455/OVJ.2022.v12.i5.15



Chicago Style

Fungfuang, Wirasak, and . "Serum proteomic analysis reveals the differential dose effects of crocodile oil from Crocodylus siamensis on energy metabolism in rats." Open Veterinary Journal 12 (2022), 697-708. doi:10.5455/OVJ.2022.v12.i5.15



MLA (The Modern Language Association) Style

Fungfuang, Wirasak, and . "Serum proteomic analysis reveals the differential dose effects of crocodile oil from Crocodylus siamensis on energy metabolism in rats." Open Veterinary Journal 12.5 (2022), 697-708. Print. doi:10.5455/OVJ.2022.v12.i5.15



APA (American Psychological Association) Style

Fungfuang, W. & (2022) Serum proteomic analysis reveals the differential dose effects of crocodile oil from Crocodylus siamensis on energy metabolism in rats. Open Veterinary Journal, 12 (5), 697-708. doi:10.5455/OVJ.2022.v12.i5.15