E-ISSN 2218-6050 | ISSN 2226-4485
 

Original Article


Generation of an inactivated vaccine for avian pathogenic Escherichia coli using microarrays; a more rational approach to inactivated vaccine design

Xiangmei Zhou, Philip Richards, Daniel Windhorst, Ariel Imre, Agnes Bukovinski, Jessica Ruggeri, Altayeb Elazomi, Paul Barrow.

Abstract
Abstract
Global gene expression in a pathogenic avian O78:K80 strain of Escherichia coli, harvested from infected avian macrophage-like HD11 cells, was compared by microarray with bacteria cultured in tissue culture medium. The changes observed included the use of different electron acceptors and carbon sources such as ethanolamine, β -glucosides, galactonate, dicarboxylic acids and amino acids, up-regulation of genes associated with Fe and Mn uptake and up-regulation of type-1 and curli fimbriae, other adhesion genes and down-regulation of sialic acid synthesis genes. This information, coupled with evidence of intracellular stress, was used to produce a synthetic liquid culture medium which aimed at reproducing the conditions in the macrophage phagosome. The E. coli strain was cultured using this medium to produce an inactivated vaccine (bacterin). This was statistically more protective than a bacterin prepared from bacteria cultured in nutrient broth when tested in vaccinated chickens challenged with a different virulent E. coli O78:K80 strain.

Key words: Key words APEC, microarray, vaccine, chicken, gene expression, synthetic medium


 
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How to Cite this Article
Pubmed Style

Zhou X, Richards P, Windhorst D, Imre A, Bukovinski A, Ruggeri J, Elazomi A, Barrow P. Generation of an inactivated vaccine for avian pathogenic Escherichia coli using microarrays; a more rational approach to inactivated vaccine design. Open Vet J. 2022; 12(2): 221-230. doi:10.5455/OVJ.2022.v12.i2.10


Web Style

Zhou X, Richards P, Windhorst D, Imre A, Bukovinski A, Ruggeri J, Elazomi A, Barrow P. Generation of an inactivated vaccine for avian pathogenic Escherichia coli using microarrays; a more rational approach to inactivated vaccine design. https://www.openveterinaryjournal.com/?mno=31285 [Access: February 07, 2023]. doi:10.5455/OVJ.2022.v12.i2.10


AMA (American Medical Association) Style

Zhou X, Richards P, Windhorst D, Imre A, Bukovinski A, Ruggeri J, Elazomi A, Barrow P. Generation of an inactivated vaccine for avian pathogenic Escherichia coli using microarrays; a more rational approach to inactivated vaccine design. Open Vet J. 2022; 12(2): 221-230. doi:10.5455/OVJ.2022.v12.i2.10



Vancouver/ICMJE Style

Zhou X, Richards P, Windhorst D, Imre A, Bukovinski A, Ruggeri J, Elazomi A, Barrow P. Generation of an inactivated vaccine for avian pathogenic Escherichia coli using microarrays; a more rational approach to inactivated vaccine design. Open Vet J. (2022), [cited February 07, 2023]; 12(2): 221-230. doi:10.5455/OVJ.2022.v12.i2.10



Harvard Style

Zhou, X., Richards, . P., Windhorst, . D., Imre, . A., Bukovinski, . A., Ruggeri, . J., Elazomi, . A. & Barrow, . P. (2022) Generation of an inactivated vaccine for avian pathogenic Escherichia coli using microarrays; a more rational approach to inactivated vaccine design. Open Vet J, 12 (2), 221-230. doi:10.5455/OVJ.2022.v12.i2.10



Turabian Style

Zhou, Xiangmei, Philip Richards, Daniel Windhorst, Ariel Imre, Agnes Bukovinski, Jessica Ruggeri, Altayeb Elazomi, and Paul Barrow. 2022. Generation of an inactivated vaccine for avian pathogenic Escherichia coli using microarrays; a more rational approach to inactivated vaccine design. Open Veterinary Journal, 12 (2), 221-230. doi:10.5455/OVJ.2022.v12.i2.10



Chicago Style

Zhou, Xiangmei, Philip Richards, Daniel Windhorst, Ariel Imre, Agnes Bukovinski, Jessica Ruggeri, Altayeb Elazomi, and Paul Barrow. "Generation of an inactivated vaccine for avian pathogenic Escherichia coli using microarrays; a more rational approach to inactivated vaccine design." Open Veterinary Journal 12 (2022), 221-230. doi:10.5455/OVJ.2022.v12.i2.10



MLA (The Modern Language Association) Style

Zhou, Xiangmei, Philip Richards, Daniel Windhorst, Ariel Imre, Agnes Bukovinski, Jessica Ruggeri, Altayeb Elazomi, and Paul Barrow. "Generation of an inactivated vaccine for avian pathogenic Escherichia coli using microarrays; a more rational approach to inactivated vaccine design." Open Veterinary Journal 12.2 (2022), 221-230. Print. doi:10.5455/OVJ.2022.v12.i2.10



APA (American Psychological Association) Style

Zhou, X., Richards, . P., Windhorst, . D., Imre, . A., Bukovinski, . A., Ruggeri, . J., Elazomi, . A. & Barrow, . P. (2022) Generation of an inactivated vaccine for avian pathogenic Escherichia coli using microarrays; a more rational approach to inactivated vaccine design. Open Veterinary Journal, 12 (2), 221-230. doi:10.5455/OVJ.2022.v12.i2.10